8HIM

A cryo-EM structure of B. oleracea RNA polymerase V elongation complex at 2.73 Angstrom

8HIM の概要

• エントリーDOI: 10.2210/pdb8him/pdb
• EMDBエントリー: 34821
• 分子名称: DNA (34-MER), DNA-directed RNA polymerases I, II, and III subunit RPABC3, DNA-directed RNA polymerase subunit, ... (15 entities in total)
• 機能のキーワード: dna-dependent rna polymerase v, transcription
• 由来する生物種: Brassica oleracea (wild cabbage); 詳細
• タンパク質・核酸の鎖数: 13
• 化学式量合計: 518698.80

構造登録者

Hu, H.,Xie, G.,Du, X.,Du, J. (登録日: 2022-11-21, 公開日: 2023-03-22, 最終更新日: 2024-07-03)

主引用文献

Xie, G.,Du, X.,Hu, H.,Li, S.,Cao, X.,Jacobsen, S.E.,Du, J.
Structure and mechanism of the plant RNA polymerase V.
Science, 379:1209-1213, 2023

PubMed Abstract: In addition to the conserved RNA polymerases I to III (Pols I to III) in eukaryotes, two atypical polymerases, Pols IV and V, specifically produce noncoding RNA in the RNA-directed DNA methylation pathway in plants. Here, we report on the structures of cauliflower Pol V in the free and elongation conformations. A conserved tyrosine residue of NRPE2 stacks with a double-stranded DNA branch of the transcription bubble to potentially attenuate elongation by inducing transcription stalling. The nontemplate DNA strand is captured by NRPE2 to enhance backtracking, thereby increasing 3'-5' cleavage, which likely underpins Pol V's high fidelity. The structures also illuminate the mechanism of Pol V transcription stalling and enhanced backtracking, which may be important for Pol V's retention on chromatin to serve its function in tethering downstream factors for RNA-directed DNA methylation.

PubMed: 36893216
DOI: 10.1126/science.adf8231

実験手法

ELECTRON MICROSCOPY (2.8 Å)