8H2H

Cryo-EM structure of a Group II Intron Complexed with its Reverse Transcriptase

Summary for 8H2H

• Entry DOI: 10.2210/pdb8h2h/pdb
• EMDB information: 33039
• Descriptor: LtrB, RNA (5'-R(P*CP*AP*CP*AP*UP*CP*CP*AP*UP*AP*AP*C)-3'), Group II intron-encoded protein LtrA (3 entities in total)
• Functional Keywords: intron, rna binding protein-rna complex, rna binding protein/rna
• Biological source: Lactococcus lactis; More
• Total number of polymer chains: 3
• Total formula weight: 365389.94

Authors

Liu, N.,Dong, X.L.,Qu, G.S.,Wang, J.,Wang, H.W.,Belfort, M. (deposition date: 2022-10-06, release date: 2022-11-23)

Primary citation

Lu, Y.,Liu, N.,Liu, Y.,Zheng, L.,Yang, J.,Wang, J.,Jia, X.,Zi, Q.,Peng, H.,Rao, Y.,Wang, H.W.
Functionalized graphene grids with various charges for single-particle cryo-EM.
Nat Commun, 13:6718-6718, 2022

PubMed Abstract: A major hurdle for single particle cryo-EM in structural determination lies in the specimen preparation impaired by the air-water interface (AWI) and preferential particle-orientation problems. In this work, we develop functionalized graphene grids with various charges via a dediazoniation reaction for cryo-EM specimen preparation. The graphene grids are paraffin-assistant fabricated, which appear with less contaminations compared with those produced by polymer transfer method. By applying onto three different types of macromolecules, we demonstrate that the high-yield charged graphene grids bring macromolecules away from the AWI and enable adjustable particle-orientation distribution for more robust single particle cryo-EM structural determination.

PubMed: 36344519
DOI: 10.1038/s41467-022-34579-w

Experimental method

ELECTRON MICROSCOPY (3.2 Å)