8GSD

Echovirus3 full particle in complex with 6D10 Fab

Summary for 8GSD

• Entry DOI: 10.2210/pdb8gsd/pdb
• EMDB information: 34231 34232 34233 34234
• Descriptor: Heavy chain of 6D10, Light chain of 6D10, Genome polyprotein (Fragment), ... (7 entities in total)
• Functional Keywords: e3 full particle, echovirus3 full particle, antibody, 6d10, virus-immune system complex, virus/immune system
• Biological source: Mus musculus; More
• Total number of polymer chains: 6
• Total formula weight: 121641.54

Authors

Wang, X.,Fu, W. (deposition date: 2022-09-06, release date: 2022-12-14, Last modification date: 2025-05-28)

Primary citation

Qi, S.,Fu, W.,Fan, J.,Zhang, L.,Zheng, B.,Wang, K.,Wang, X.,Zhu, L.,Li, X.,Zhang, Y.
Structural Basis for the Immunogenicity of the C-Terminus of VP1 of Echovirus 3 Revealed by the Binding of a Neutralizing Antibody.
Viruses, 14:-, 2022

PubMed Abstract: Echovirus 3 (E3), a serotype of human enterovirus B (HEV-B), causes severe diseases in infants. Here, we determined the structures of E3 with a monoclonal antibody (MAb) 6D10 by cryo-EM to comprehensively understand the specificities and the immunological characteristic of this serotype. The solved cryo-EM structures of the F-, A-, and E-particles of E3 bound with 6D10 revealed the structural features of the virus-antibody interface. Importantly, the structures of E-particles bound with 6D10 revealed for the first time the nature of the C-terminus of VP1 for HEV-Bs at the structural level. The highly immunogenic nature of this region in the E-particles provides new strategies for vaccine development for HEV-Bs.

PubMed: 36366420
DOI: 10.3390/v14112322

Experimental method

ELECTRON MICROSCOPY (3.1 Å)