8GOS

Crystal structure of fluorescent protein RasM

Summary for 8GOS

• Entry DOI: 10.2210/pdb8gos/pdb
• Descriptor: RasM (2 entities in total)
• Functional Keywords: chromophore, monomer, mutant, fluorescent protein
• Biological source: Discosoma sp.
• Total number of polymer chains: 2
• Total formula weight: 51628.68

Authors

Adachi, M.,Kagotani, Y.,Shimizu, R. (deposition date: 2022-08-25, release date: 2023-09-27, Last modification date: 2023-11-15)

Primary citation

Tsubouchi, M.,Ishii, N.,Kagotani, Y.,Shimizu, R.,Fujita, T.,Adachi, M.,Itakura, R.
Beat-frequency-resolved two-dimensional electronic spectroscopy: disentangling vibrational coherences in artificial fluorescent proteins with sub-10-fs visible laser pulses.
Opt Express, 31:6890-6906, 2023

PubMed Abstract: We perform a beat-frequency-resolved analysis for two-dimensional electronic spectroscopy using a high-speed and stable 2D electronic spectrometer and few-cycle visible laser pulses to disentangle the vibrational coherences in an artificial fluorescent protein. We develop a highly stable ultrashort light source that generates 5.3-fs visible pulses with a pulse energy of 4.7 µJ at a repetition rate of 10 kHz using multi-plate pulse compression and laser filamentation in a gas cell. The above-5.3-fs laser pulses together with a high-speed multichannel detector enable us to measure a series of 2D electronic spectra, which are resolved in terms of beat frequency related to vibrational coherence. We successfully extract the discrete vibrational peaks behind the inhomogeneous broadening in the absorption spectra and the vibrational quantum beats of the excited electronic state behind the strong incoherent population background in the typical 2D electronic spectra.

PubMed: 36823935
DOI: 10.1364/OE.480505

Experimental method

X-RAY DIFFRACTION (1.01 Å)