8GMO

Bacteriophage T4 capsid shell containing 9DE insertions into the gp23* major capsid protein subunits

これはPDB形式変換不可エントリーです。

8GMO の概要

• エントリーDOI: 10.2210/pdb8gmo/pdb
• EMDBエントリー: 40228
• 分子名称: Mature major capsid protein, Mature capsid vertex protein (2 entities in total)
• 機能のキーワード: bacteriophage t4, capsid shell, phage display, decorated capsid, phage head, virus like particle
• 由来する生物種: Tequatrovirus T4; 詳細
• タンパク質・核酸の鎖数: 98
• 化学式量合計: 4858509.49

構造登録者

Fokine, A.,Rao, V.B. (登録日: 2023-03-26, 公開日: 2023-05-10, 最終更新日: 2024-06-19)

主引用文献

Zhu, J.,Batra, H.,Ananthaswamy, N.,Mahalingam, M.,Tao, P.,Wu, X.,Guo, W.,Fokine, A.,Rao, V.B.
Design of bacteriophage T4-based artificial viral vectors for human genome remodeling.
Nat Commun, 14:2928-2928, 2023

PubMed Abstract: Designing artificial viral vectors (AVVs) programmed with biomolecules that can enter human cells and carry out molecular repairs will have broad applications. Here, we describe an assembly-line approach to build AVVs by engineering the well-characterized structural components of bacteriophage T4. Starting with a 120 × 86 nm capsid shell that can accommodate 171-Kbp DNA and thousands of protein copies, various combinations of biomolecules, including DNAs, proteins, RNAs, and ribonucleoproteins, are externally and internally incorporated. The nanoparticles are then coated with cationic lipid to enable efficient entry into human cells. As proof of concept, we assemble a series of AVVs designed to deliver full-length dystrophin gene or perform various molecular operations to remodel human genome, including genome editing, gene recombination, gene replacement, gene expression, and gene silencing. These large capacity, customizable, multiplex, and all-in-one phage-based AVVs represent an additional category of nanomaterial that could potentially transform gene therapies and personalized medicine.

PubMed: 37253769
DOI: 10.1038/s41467-023-38364-1

実験手法

ELECTRON MICROSCOPY (3.9 Å)