8GH6

Bombyx mori R2 retrotransposon initiating target-primed reverse transcription

これはPDB形式変換不可エントリーです。

8GH6 の概要

• エントリーDOI: 10.2210/pdb8gh6/pdb
• EMDBエントリー: 40033
• 分子名称: Reverse transcriptase-like protein, 28S DNA bottom strand, 3' side, 28S DNA bottom strand, 5' side (priming strand), ... (8 entities in total)
• 機能のキーワード: retrotransposon, line, reverse transcriptase, endonuclease, rna binding protein, rna binding protein-rna-dna complex, rna binding protein/rna/dna
• 由来する生物種: Bombyx mori (domestic silkworm); 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 255812.02

構造登録者

Wilkinson, M.E.,Zhang, F. (登録日: 2023-03-09, 公開日: 2023-04-05, 最終更新日: 2023-08-16)

主引用文献

Wilkinson, M.E.,Frangieh, C.J.,Macrae, R.K.,Zhang, F.
Structure of the R2 non-LTR retrotransposon initiating target-primed reverse transcription.
Science, 380:301-308, 2023

PubMed Abstract: Non-long terminal repeat (non-LTR) retrotransposons, or long interspersed nuclear elements (LINEs), are an abundant class of eukaryotic transposons that insert into genomes by target-primed reverse transcription (TPRT). During TPRT, a target DNA sequence is nicked and primes reverse transcription of the retrotransposon RNA. Here, we report the cryo-electron microscopy structure of the R2 non-LTR retrotransposon initiating TPRT at its ribosomal DNA target. The target DNA sequence is unwound at the insertion site and recognized by an upstream motif. An extension of the reverse transcriptase (RT) domain recognizes the retrotransposon RNA and guides the 3' end into the RT active site to template reverse transcription. We used Cas9 to retarget R2 in vitro to non-native sequences, suggesting future use as a reprogrammable RNA-based gene-insertion tool.

PubMed: 37023171
DOI: 10.1126/science.adg7883

実験手法

ELECTRON MICROSCOPY (3.08 Å)