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8GGG

RNase A-Adenosine 5'-Hexaphosphate (RNaseA.p6A)

Summary for 8GGG
Entry DOI10.2210/pdb8ggg/pdb
DescriptorRibonuclease pancreatic, adenosine 5'-hexaphosphate, GLYCEROL, ... (4 entities in total)
Functional Keywordsrnase a, oligophosphate, inhibitor, nucleoside hexaphosphate, protein-ligand complex, rna binding protein-inhibitor complex, rna binding protein/inhibitor
Biological sourceBos taurus (cattle)
Total number of polymer chains2
Total formula weight29934.30
Authors
Park, G.,Cummins, C. (deposition date: 2023-03-08, release date: 2024-03-13, Last modification date: 2024-10-30)
Primary citationPark, G.,Wralstad, E.C.,Faginas-Lago, N.,Qian, K.,Raines, R.T.,Bistoni, G.,Cummins, C.C.
Pentaphosphorylation via the Anhydride of Dihydrogen Pentametaphosphate: Access to Nucleoside Hexa- and Heptaphosphates and Study of Their Interaction with Ribonuclease A.
Acs Cent.Sci., 10:1415-1422, 2024
Cited by
PubMed Abstract: Pentametaphosphate is the little studied cyclic pentamer of the metaphosphate ion, [PO] . We show that the doubly protonated form of this pentamer can be selectively dehydrated to provide the anhydride [PO] (). This trianion is the well-defined condensed phosphate component of a novel reagent for attachment of a pentaphosphate chain to biomolecules all in one go. Here, we demonstrate by extending adenosine monophosphate (AMP) and uridine monophosphate (UMP) to their corresponding nucleoside hexaphosphates, while adenosine diphosphate (ADP) and uridine diphosphate (UDP) are phosphate chain-extended to the corresponding nucleoside heptaphosphates. Such constructs are of interest for their potential biological function with respect to RNA-processing enzymes. Thus, we go on to investigate in detail the interaction of the polyanionic constructs with ribonuclease A, a model protein containing a polycationic active site and for which X-ray crystal structures are relatively straightforward to obtain. This work presents a combined experimental and quantum chemical approach to understanding the interactions of RNase A with the new nucleoside hexa- and heptaphosphate constructs.
PubMed: 39071052
DOI: 10.1021/acscentsci.4c00835
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.86 Å)
Structure validation

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PDB entries from 2024-11-13

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