8G92

Structure of inhibitor 16d-bound SPNS2

Summary for 8G92

• Entry DOI: 10.2210/pdb8g92/pdb
• EMDB information: 29860
• Descriptor: Sphingosine-1-phosphate transporter SPNS2, 3-[3-(4-decylphenyl)-1,2,4-oxadiazol-5-yl]propan-1-amine (2 entities in total)
• Functional Keywords: spns2, sphingosine-1-phosphate, major facilitator superfamily, transporter, immune system
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 50113.65

Authors

Chen, H.,Li, X. (deposition date: 2023-02-21, release date: 2023-05-24, Last modification date: 2023-12-13)

Primary citation

Chen, H.,Ahmed, S.,Zhao, H.,Elghobashi-Meinhardt, N.,Dai, Y.,Kim, J.H.,McDonald, J.G.,Li, X.,Lee, C.H.
Structural and functional insights into Spns2-mediated transport of sphingosine-1-phosphate.
Cell, 186:2644-2655.e16, 2023

PubMed Abstract: Sphingosine-1-phosphate (S1P) is an important signaling sphingolipid that regulates the immune system, angiogenesis, auditory function, and epithelial and endothelial barrier integrity. Spinster homolog 2 (Spns2) is an S1P transporter that exports S1P to initiate lipid signaling cascades. Modulating Spns2 activity can be beneficial in treatments of cancer, inflammation, and immune diseases. However, the transport mechanism of Spns2 and its inhibition remain unclear. Here, we present six cryo-EM structures of human Spns2 in lipid nanodiscs, including two functionally relevant intermediate conformations that link the inward- and outward-facing states, to reveal the structural basis of the S1P transport cycle. Functional analyses suggest that Spns2 exports S1P via facilitated diffusion, a mechanism distinct from other MFS lipid transporters. Finally, we show that the Spns2 inhibitor 16d attenuates the transport activity by locking Spns2 in the inward-facing state. Our work sheds light on Spns2-mediated S1P transport and aids the development of advanced Spns2 inhibitors.

PubMed: 37224812
DOI: 10.1016/j.cell.2023.04.028

Experimental method

ELECTRON MICROSCOPY (3.6 Å)