8G4E

Green Fluorescence Protein imaged on a cryo-EM imaging scaffold

Summary for 8G4E

Entry DOI	10.2210/pdb8g4e/pdb
EMDB information	29718
Descriptor	RCG-10 - Cryo-EM imaging scaffold subunit B fused to DARPin, Superfolder Green Fluorescent Protein (2 entities in total)
Functional Keywords	cryoem imaging scaffold, cancer, gtpase, signaling protein
Biological source	synthetic construct More
Total number of polymer chains	2
Total formula weight	61911.16
Authors	Castells-Graells, R.,Sawaya, M.R.,Yeates, T.O. (deposition date: 2023-02-09, release date: 2023-08-09, Last modification date: 2023-11-15)
Primary citation	Castells-Graells, R.,Meador, K.,Arbing, M.A.,Sawaya, M.R.,Gee, M.,Cascio, D.,Gleave, E.,Debreczeni, J.E.,Breed, J.,Leopold, K.,Patel, A.,Jahagirdar, D.,Lyons, B.,Subramaniam, S.,Phillips, C.,Yeates, T.O. Cryo-EM structure determination of small therapeutic protein targets at 3 angstrom -resolution using a rigid imaging scaffold. Proc.Natl.Acad.Sci.USA, 120:e2305494120-e2305494120, 2023 Cited by PubMed: 37669364 DOI: 10.1073/pnas.2305494120 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (2.98 Å)

Structure validation