8G3D

48-nm doublet microtubule from Tetrahymena thermophila strain K40R

これはPDB形式変換不可エントリーです。

8G3D の概要

• エントリーDOI: 10.2210/pdb8g3d/pdb
• 関連するPDBエントリー: 8G2Z
• EMDBエントリー: 29666 29667 29685 29692
• 分子名称: RIB27A, CFAP20, Parkin co-regulated protein PACRGA, ... (49 entities in total)
• 機能のキーワード: cilia, axoneme, doublet microtubule, microtubule inner protein, structural protein
• 由来する生物種: Tetrahymena thermophila; 詳細
• タンパク質・核酸の鎖数: 431
• 化学式量合計: 20362468.49

構造登録者

Black, C.S.,Kubo, S.,Yang, S.K.,Bui, K.H. (登録日: 2023-02-07, 公開日: 2023-06-14, 最終更新日: 2024-10-30)

主引用文献

Kubo, S.,Black, C.S.,Joachimiak, E.,Yang, S.K.,Legal, T.,Peri, K.,Khalifa, A.A.Z.,Ghanaeian, A.,McCafferty, C.L.,Valente-Paterno, M.,De Bellis, C.,Huynh, P.M.,Fan, Z.,Marcotte, E.M.,Wloga, D.,Bui, K.H.
Native doublet microtubules from Tetrahymena thermophila reveal the importance of outer junction proteins.
Nat Commun, 14:2168-2168, 2023

PubMed Abstract: Cilia are ubiquitous eukaryotic organelles responsible for cellular motility and sensory functions. The ciliary axoneme is a microtubule-based cytoskeleton consisting of two central singlets and nine outer doublet microtubules. Cryo-electron microscopy-based studies have revealed a complex network inside the lumen of both tubules composed of microtubule-inner proteins (MIPs). However, the functions of most MIPs remain unknown. Here, we present single-particle cryo-EM-based analyses of the Tetrahymena thermophila native doublet microtubule and identify 42 MIPs. These data shed light on the evolutionarily conserved and diversified roles of MIPs. In addition, we identified MIPs potentially responsible for the assembly and stability of the doublet outer junction. Knockout of the evolutionarily conserved outer junction component CFAP77 moderately diminishes Tetrahymena swimming speed and beat frequency, indicating the important role of CFAP77 and outer junction stability in cilia beating generation and/or regulation.

PubMed: 37061538
DOI: 10.1038/s41467-023-37868-0

実験手法

ELECTRON MICROSCOPY (3.7 Å)