8FRU

60S subunit of the Giardia lamblia 80S ribosome

これはPDB形式変換不可エントリーです。

8FRU の概要

• エントリーDOI: 10.2210/pdb8fru/pdb
• 関連するPDBエントリー: 8FVY
• EMDBエントリー: 29407
• 分子名称: 60S ribosomal protein uL3, 60S ribosomal protein uL13, 60S ribosomal protein eL13, ... (49 entities in total)
• 機能のキーワード: giardia lamblia, ribosome structure, translation, parasite, ribosome
• 由来する生物種: Giardia intestinalis assemblage A; 詳細
• タンパク質・核酸の鎖数: 43
• 化学式量合計: 1687716.98

構造登録者

Eiler, D.R.,Wimberly, B.T.,Bilodeau, D.Y.,Rissland, O.S.,Kieft, J.S. (登録日: 2023-01-08, 公開日: 2024-01-31, 最終更新日: 2024-04-17)

主引用文献

Eiler, D.R.,Wimberly, B.T.,Bilodeau, D.Y.,Taliaferro, J.M.,Reigan, P.,Rissland, O.S.,Kieft, J.S.
The Giardia lamblia ribosome structure reveals divergence in several biological pathways and the mode of emetine function.
Structure, 32:400-, 2024

PubMed Abstract: Giardia lamblia is a deeply branching protist and a human pathogen. Its unusual biology presents the opportunity to explore conserved and fundamental molecular mechanisms. We determined the structure of the G. lamblia 80S ribosome bound to tRNA, mRNA, and the antibiotic emetine by cryo-electron microscopy, to an overall resolution of 2.49 Å. The structure reveals rapidly evolving protein and nucleotide regions, differences in the peptide exit tunnel, and likely altered ribosome quality control pathways. Examination of translation initiation factor binding sites suggests these interactions are conserved despite a divergent initiation mechanism. Highlighting the potential of G. lamblia to resolve conserved biological principles; our structure reveals the interactions of the translation inhibitor emetine with the ribosome and mRNA, thus providing insight into the mechanism of action for this widely used antibiotic. Our work defines key questions in G. lamblia and motivates future experiments to explore the diversity of eukaryotic gene regulation.

PubMed: 38242118
DOI: 10.1016/j.str.2023.12.015

実験手法

ELECTRON MICROSCOPY (2.49 Å)