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- EMDB-29407: 60S subunit of the Giardia lamblia 80S ribosome -

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Basic information

Entry
Database: EMDB / ID: EMD-29407
Title60S subunit of the Giardia lamblia 80S ribosome
Map data
Sample
  • Complex: 60S ribosomal subunit of the 80S Giardia intestinalis assemblage A ribosome
    • Protein or peptide: x 40 types
    • RNA: x 3 types
  • Ligand: x 6 types
KeywordsGiardia lamblia / Ribosome structure / Translation / parasite / RIBOSOME
Function / homology
Function and homology information


maturation of LSU-rRNA / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / modification-dependent protein catabolic process / protein tag activity / large ribosomal subunit / ribosome biogenesis / 5S rRNA binding / large ribosomal subunit rRNA binding / ribosomal large subunit assembly ...maturation of LSU-rRNA / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / modification-dependent protein catabolic process / protein tag activity / large ribosomal subunit / ribosome biogenesis / 5S rRNA binding / large ribosomal subunit rRNA binding / ribosomal large subunit assembly / cytoplasmic translation / cytosolic large ribosomal subunit / negative regulation of translation / rRNA binding / ribosome / protein ubiquitination / structural constituent of ribosome / ribonucleoprotein complex / translation / mRNA binding / ubiquitin protein ligase binding / nucleolus / RNA binding / metal ion binding
Similarity search - Function
Ribosomal protein L40e, archaeal / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. / : / Ribosomal protein L18e / Ribosomal protein 60S L18 and 50S L18e / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. ...Ribosomal protein L40e, archaeal / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. / : / Ribosomal protein L18e / Ribosomal protein 60S L18 and 50S L18e / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein L10e / Ribosomal protein L13e / Ribosomal protein L13e / 60S ribosomal protein L18a/ L20, eukaryotes / : / Ribosomal protein L44e / Ribosomal protein L44 / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal L40e family / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal L27e protein family / Ribosomal protein L34Ae / Ribosomal protein L34e / Ribosomal protein L14e domain / Ribosomal protein L35Ae, conserved site / 60S ribosomal protein L19 / Ribosomal protein L14 / Ribosomal protein L35Ae signature. / Ribosomal protein L30/YlxQ / Ribosomal protein L7A/L8 / Ribosomal protein L13, eukaryotic/archaeal / 60S ribosomal protein L35 / Ribosomal protein L14 / Ribosomal protein L14, KOW motif / Ribosomal protein L37ae / Ribosomal L37ae protein family / 60S ribosomal protein L4, C-terminal domain / 60S ribosomal protein L4 C-terminal domain / Ribosomal protein L31e, conserved site / Ribosomal protein L31e signature. / Ribosomal_L19e / Ribosomal protein L19/L19e / Ribosomal protein L19/L19e, domain 1 / Ribosomal protein L19/L19e superfamily / Ribosomal protein L19e / Ribosomal protein L36e / Ribosomal protein L36e domain superfamily / Ribosomal protein L36e / Ribosomal protein L35A / Ribosomal protein L35Ae / Ribosomal protein L5 eukaryotic/L18 archaeal / Ribosomal large subunit proteins 60S L5, and 50S L18 / Ribosomal protein L35A superfamily / Ribosomal protein L32e, conserved site / Ribosomal protein L32e signature. / Ribosomal protein L4/L1e, eukaryotic/archaeal, conserved site / Ribosomal protein L1e signature. / Ribosomal protein L15e, conserved site / Ribosomal protein L15e signature. / Ribosomal protein L31e / Ribosomal protein L31e domain superfamily / Ribosomal protein L31e / Ribosomal_L31e / Ribosomal protein L21e / Ribosomal protein L21e, conserved site / Ribosomal protein L21 superfamily / Ribosomal protein L21e / Ribosomal protein L21e signature. / Ribosomal protein L37e, conserved site / Ribosomal protein L37e signature. / Ribosomal protein L24e-related / Ribosomal protein L24e/L24 superfamily / Ribosomal protein L24e / Ribosomal protein L37e / Ribosomal protein L22/L17, eukaryotic/archaeal / Ribosomal protein L37e / Ribosomal protein L4, eukaryotic and archaeal type / Ribosomal_L15e / Ribosomal protein L15e / Ribosomal protein L15e core domain superfamily / Ribosomal protein L3, domain 3, archaeal type superfamily
Similarity search - Domain/homology
LSU ribosomal protein L29P/L35 / Ribosomal protein L27 / Ribosomal protein L21 / Ribosomal L38e / Ribosomal protein L14 / Ribosomal protein L18 / Ribosomal protein L30 / 60S ribosomal protein L13 / Ribosomal protein L4 / Ribosomal protein L15 ...LSU ribosomal protein L29P/L35 / Ribosomal protein L27 / Ribosomal protein L21 / Ribosomal L38e / Ribosomal protein L14 / Ribosomal protein L18 / Ribosomal protein L30 / 60S ribosomal protein L13 / Ribosomal protein L4 / Ribosomal protein L15 / Ribosomal protein L17 / Ribosomal protein L19 / Ribosomal protein L11 / Ribosomal protein L35a / 60S ribosomal protein L7a / Ribosomal protein L10 / Ribosomal protein L32 / Large ribosomal subunit protein eL34 / Ribosomal protein L31e / Ribosomal protein L23A / Ribosomal protein L37a / Ribosomal protein L6 / Ribosomal protein L44 / Ribosomal protein L24A / Ribosomal protein L7 / Ribosomal protein L23 / Ribosomal protein L13a / Large ribosomal subunit protein eL22 / 50S ribosomal protein L40e / Ribosomal protein L36e / Ribosomal protein L27a / Ribosomal protein L8 / Ribosomal protein L5 / 60S ribosomal protein L29 / LSU ribosomal protein L18AE / LSU ribosomal protein L24P / LSU ribosomal protein L3P / Ribosomal protein L37
Similarity search - Component
Biological speciesGiardia intestinalis assemblage A (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.49 Å
AuthorsEiler DR / Wimberly BT / Bilodeau DY / Rissland OS / Kieft JS
Funding support United States, 3 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35GM118070 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)1R21AI149210 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35GM128680 United States
CitationJournal: Structure / Year: 2024
Title: The Giardia lamblia ribosome structure reveals divergence in several biological pathways and the mode of emetine function.
Authors: Daniel R Eiler / Brian T Wimberly / Danielle Y Bilodeau / J Matthew Taliaferro / Philip Reigan / Olivia S Rissland / Jeffrey S Kieft /
Abstract: Giardia lamblia is a deeply branching protist and a human pathogen. Its unusual biology presents the opportunity to explore conserved and fundamental molecular mechanisms. We determined the structure ...Giardia lamblia is a deeply branching protist and a human pathogen. Its unusual biology presents the opportunity to explore conserved and fundamental molecular mechanisms. We determined the structure of the G. lamblia 80S ribosome bound to tRNA, mRNA, and the antibiotic emetine by cryo-electron microscopy, to an overall resolution of 2.49 Å. The structure reveals rapidly evolving protein and nucleotide regions, differences in the peptide exit tunnel, and likely altered ribosome quality control pathways. Examination of translation initiation factor binding sites suggests these interactions are conserved despite a divergent initiation mechanism. Highlighting the potential of G. lamblia to resolve conserved biological principles; our structure reveals the interactions of the translation inhibitor emetine with the ribosome and mRNA, thus providing insight into the mechanism of action for this widely used antibiotic. Our work defines key questions in G. lamblia and motivates future experiments to explore the diversity of eukaryotic gene regulation.
History
DepositionJan 8, 2023-
Header (metadata) releaseJan 31, 2024-
Map releaseJan 31, 2024-
UpdateApr 17, 2024-
Current statusApr 17, 2024Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_29407.png

Downloads & links

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Map

FileDownload / File: emd_29407.map.gz / Format: CCP4 / Size: 347.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.82 Å/pix.
x 450 pix.
= 369.495 Å		0.82 Å/pix.
x 450 pix.
= 369.495 Å		0.82 Å/pix.
x 450 pix.
= 369.495 Å

Surface

Projections

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Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.8211 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.461
Minimum - Maximum-1.459785 - 3.9904838
Average (Standard dev.)-0.0012536786 (±0.15223372)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions450450450
Spacing450450450
CellA=B=C: 369.495 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_29407_half_map_1.map
Projections & Slices
AxesZYX

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Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_29407_half_map_2.map
Projections & Slices
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Histogram (log scale)

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Sample components

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Entire : 60S ribosomal subunit of the 80S Giardia intestinalis assemblage ...

EntireName: 60S ribosomal subunit of the 80S Giardia intestinalis assemblage A ribosome
Components
  • Complex: 60S ribosomal subunit of the 80S Giardia intestinalis assemblage A ribosome
    • Protein or peptide: 60S ribosomal protein uL3
    • Protein or peptide: 60S ribosomal protein uL15
    • Protein or peptide: 60S ribosomal protein uL4
    • Protein or peptide: 60S ribosomal protein uL16
    • Protein or peptide: 60S ribosomal protein eL43
    • Protein or peptide: 60S ribosomal protein uL18
    • Protein or peptide: 60S ribosomal protein uL6
    • Protein or peptide: 60S ribosomal protein eL8
    • Protein or peptide: 60S ribosomal protein uL5
    • Protein or peptide: 60S ribosomal protein uL13
    • Protein or peptide: 60S ribosomal protein eL13
    • Protein or peptide: 60S ribosomal protein eL14
    • Protein or peptide: 60S ribosomal protein eL15
    • Protein or peptide: 60S ribosomal protein eL18
    • Protein or peptide: 60S ribosomal protein eL20
    • Protein or peptide: 60S ribosomal protein eL19
    • Protein or peptide: 60S ribosomal protein eL21
    • Protein or peptide: 60S ribosomal protein uL14
    • Protein or peptide: 60S ribosomal protein uL22
    • Protein or peptide: 60S ribosomal protein uL23
    • Protein or peptide: 60S ribosomal protein uL24
    • Protein or peptide: 60S ribosomal protein eL24
    • Protein or peptide: 60S ribosomal protein eL27
    • Protein or peptide: 60S ribosomal protein eL29
    • Protein or peptide: 60S ribosomal protein uL29
    • Protein or peptide: 60S ribosomal protein uL30
    • Protein or peptide: 60S ribosomal protein eL31
    • Protein or peptide: 60S ribosomal protein eL32
    • Protein or peptide: 60S ribosomal protein eL34
    • Protein or peptide: 60S ribosomal protein eL33
    • Protein or peptide: 60S ribosomal protein eL36
    • Protein or peptide: 60S ribosomal protein eL38
    • Protein or peptide: 60S ribosomal protein eL42
    • Protein or peptide: 60S ribosomal protein eL22
    • Protein or peptide: 60S ribosomal protein eL30
    • Protein or peptide: 60S ribosomal protein eL37
    • Protein or peptide: 60S ribosomal protein eL39
    • Protein or peptide: 60S ribosomal protein eL40
    • Protein or peptide: 60S ribosomal protein uL2
    • Protein or peptide: 60S ribosomal protein eL41
    • RNA: 5S rRNA
    • RNA: 5.8S rRNA
    • RNA: 28S rRNA
  • Ligand: POTASSIUM ION
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION
  • Ligand: SODIUM ION
  • Ligand: SPERMIDINE
  • Ligand: water

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Supramolecule #1: 60S ribosomal subunit of the 80S Giardia intestinalis assemblage ...

SupramoleculeName: 60S ribosomal subunit of the 80S Giardia intestinalis assemblage A ribosome
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#43
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)

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Macromolecule #1: 60S ribosomal protein uL3

MacromoleculeName: 60S ribosomal protein uL3 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 42.68852 KDa
SequenceString: MSHRKFSCCR KGNLGYLPRK RCTRGRGRCK TFPPDVQGAP IHLTAFLGYK AGCTHVMRFI DHRGSNLHNR QAIDQATIID APPMICTAI IGYAKTPKGL RAVTTVWAAH IAEPAMRRFY RNYFHAEKTA FSTYMKKAAD GTYIKEQLGR LKQHADVIRI V AHTQPALT ...String:
MSHRKFSCCR KGNLGYLPRK RCTRGRGRCK TFPPDVQGAP IHLTAFLGYK AGCTHVMRFI DHRGSNLHNR QAIDQATIID APPMICTAI IGYAKTPKGL RAVTTVWAAH IAEPAMRRFY RNYFHAEKTA FSTYMKKAAD GTYIKEQLGR LKQHADVIRI V AHTQPALT PLKQKKADIM EIQVNGGASV AEKVDYAYAL MEKPISIKDV FSVGAQIDTI SITRGRGFEG VVTRWGVTRL PR KTRRGNR KVACIGAWHP ANVQYTVARA GQMGYFHRTD TNKQVFMIDT AENPRCCTTE FDLTDKSINP VGGFVNYGRV QGD FIMIKG TCPGPKRRPV VMRNALLPHR NCPPVQLQWI STASKFGHGI FETVEERRQF YAK

UniProtKB: LSU ribosomal protein L3P

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Macromolecule #2: 60S ribosomal protein uL15

MacromoleculeName: 60S ribosomal protein uL15 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 17.166957 KDa
SequenceString:
MPTRIRKSRK RRGHRQMGYG RVGGHRKHPS GRGKAGGLQH KRTSFDKYHP GYFGKLGMRN YHVRRNIEYN PTINIDKIWS LIPEEQRDG FLQRKSTTEA PVVDVTRFGF FKVLGRGSLP QCPIIIKARY FSSDAEKKLR EVGGVPLLVP

UniProtKB: Ribosomal protein L27a

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Macromolecule #3: 60S ribosomal protein uL4

MacromoleculeName: 60S ribosomal protein uL4 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 35.048707 KDa
SequenceString: MNPTVKVFGP TGTQVAELPR PKVFNVPLRP DIINFVHTQL RKCLRTPYAV SRYAGVQCTA HSWGPGRAVA RLPRKHGGIG AYANFARGG HMAHPTTVIR RWCRKVNLNQ RRYAVASALA ASANAALVEA RGHRIADVPS IPLVVDTDNV TKTKDALAII K AVGVLRDV ...String:
MNPTVKVFGP TGTQVAELPR PKVFNVPLRP DIINFVHTQL RKCLRTPYAV SRYAGVQCTA HSWGPGRAVA RLPRKHGGIG AYANFARGG HMAHPTTVIR RWCRKVNLNQ RRYAVASALA ASANAALVEA RGHRIADVPS IPLVVDTDNV TKTKDALAII K AVGVLRDV ERCKDSRHIR AGRGKMRNRR YITRKGPLVI YNTEDVARGF RNLPGVDLCH VSSIRLLELA PGSHPGRLIL WT KSAFASL DDVYAAKQNY TLPRSCITQT DIERILQSDA VQKTFVPKRD PLVIERKVDP FSSKEALARL DPSGTVMPK

UniProtKB: Ribosomal protein L4

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Macromolecule #4: 60S ribosomal protein uL16

MacromoleculeName: 60S ribosomal protein uL16 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 24.283172 KDa
SequenceString: MGRRPARCYR YQKNKPYPKS RFCRGVPDPK IRYFDLGNRR AKVNDFPYCF HLLSGEKEQV SSEALEACRI ACNKYIAKKA GKDSFHLRI RVHPFHVIRI NKMLSCAGAD RLQTGMRGAW GKPQGTVARV SIGQPLLSVR CRASAKDYVK DALRRAKFKI P GRQAIVES ...String:
MGRRPARCYR YQKNKPYPKS RFCRGVPDPK IRYFDLGNRR AKVNDFPYCF HLLSGEKEQV SSEALEACRI ACNKYIAKKA GKDSFHLRI RVHPFHVIRI NKMLSCAGAD RLQTGMRGAW GKPQGTVARV SIGQPLLSVR CRASAKDYVK DALRRAKFKI P GRQAIVES RNWGFTEFTK EEYEDLRERG ELQYDGNNAH RISRKGPLNL KL

UniProtKB: Ribosomal protein L10

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Macromolecule #5: 60S ribosomal protein eL43

MacromoleculeName: 60S ribosomal protein eL43 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 10.508366 KDa
SequenceString:
MAKRTKKVGL TGKYGTRYGR AARKQLLKIE TSQKAKYTCP FCAKDKVRRT ACGIWTCRSC SKTMAGGAYS LHTEPANAAR ATIRRLRLA REDAQ

UniProtKB: Ribosomal protein L37a

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Macromolecule #6: 60S ribosomal protein uL18

MacromoleculeName: 60S ribosomal protein uL18 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 33.961375 KDa
SequenceString: MGWVKVQKTR SYFKRFQVQF RRRREGKTDY QARKALIIQD KNKYATPRYR FVVRRTNKDI ICQVTAAELT KDRVVCAAYS HELPRYGIP VGLTNYAAAY ATGLLCGRRL LEKLSVGIKE NALAQLYPGL DKATGEEYHP DENENEEVHA FKAFLDIGLA R ASTGARVF ...String:
MGWVKVQKTR SYFKRFQVQF RRRREGKTDY QARKALIIQD KNKYATPRYR FVVRRTNKDI ICQVTAAELT KDRVVCAAYS HELPRYGIP VGLTNYAAAY ATGLLCGRRL LEKLSVGIKE NALAQLYPGL DKATGEEYHP DENENEEVHA FKAFLDIGLA R ASTGARVF AAMKGAVDAG LNIPHSMKRF PGYSKDGFDS SALRDRIMGK HVGDYMAELK AEDEETYRKR FSKYIAAKVE PL RLEAMYT KAHAAIRANP QAVKAKFEIK KPVRQARLTL KERKERIDAK KLEALKQLTK

UniProtKB: Ribosomal protein L5

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Macromolecule #7: 60S ribosomal protein uL6

MacromoleculeName: 60S ribosomal protein uL6 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 20.889379 KDa
SequenceString:
MPLCSSMTIK VPTDVECTIN KRVVTVKGKK GTLVRDFSHA PLDITHANDT ISVAVWFPRG KRNALPRTIC SHIENMFKGV TYGFEYKMR LAYAHFPIAA TVVDNNKAIE IRNFMHQIKT RRIECLPGVT IAMSTNVKDE LILRGISIED VSHTAARIHE S LKVPNKDL RKFLDGCYVS SRGLQEQ

UniProtKB: Ribosomal protein L6

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Macromolecule #8: 60S ribosomal protein eL8

MacromoleculeName: 60S ribosomal protein eL8 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 25.427725 KDa
SequenceString: MSKVSGSDIK RALAVPENKS RSKCDFDLTP FVRWPRQVRI QRQKAVLQRR LKVPPTVNQF MNPISRNLTN EIFNLARKYS PESKEEHKA RLLQIADAKA NGKPLPEKSD KLVIASGIRR ITSLVESKRA KLVLIANDVD PLELVLWLPT LCHKMGVPYA I VRTKGDLG ...String:
MSKVSGSDIK RALAVPENKS RSKCDFDLTP FVRWPRQVRI QRQKAVLQRR LKVPPTVNQF MNPISRNLTN EIFNLARKYS PESKEEHKA RLLQIADAKA NGKPLPEKSD KLVIASGIRR ITSLVESKRA KLVLIANDVD PLELVLWLPT LCHKMGVPYA I VRTKGDLG KLVHLKKTTS VCFTDVNPED KPTFDKILAA VAHEVDYAKA MKTYGGGVRR EDEAQQM

UniProtKB: 60S ribosomal protein L7a

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Macromolecule #9: 60S ribosomal protein uL5

MacromoleculeName: 60S ribosomal protein uL5 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 19.767684 KDa
SequenceString:
MSQESENPMR RIRIEKLVLN ICIGGEGDAL TKAAKVLQQL TDQEPTFSKS RLTIRGFGIR RGQKISTHVT VRGEKAREII ERGLRVKNF ELPYSCFSTT GNFGFGITEH IDLGIKYDPS MGIYGMDFYV VLSRPGFRVN KRRAQQQHVG ASHKITPDEA R AWVEREFE VVYTK

UniProtKB: Ribosomal protein L11

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Macromolecule #10: 60S ribosomal protein uL13

MacromoleculeName: 60S ribosomal protein uL13 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 22.921914 KDa
SequenceString: MSRIVIDCKG HILGRLSAIV AKELLHGHEV ALVRCEKAII SGAFRDVKAH YLKKMHKRTN FNHLRGPFHF RAPAEIVRRT IRGMIPYKT VRGSDAMSRL RVYDGIPPCC ARYKRVVCPS AMAYLCLRPE RRRCEVGRVA HEIGWQHAAD VERYEKRRLE E CDKWYQDK ...String:
MSRIVIDCKG HILGRLSAIV AKELLHGHEV ALVRCEKAII SGAFRDVKAH YLKKMHKRTN FNHLRGPFHF RAPAEIVRRT IRGMIPYKT VRGSDAMSRL RVYDGIPPCC ARYKRVVCPS AMAYLCLRPE RRRCEVGRVA HEIGWQHAAD VERYEKRRLE E CDKWYQDK IQIDKKIAEA AEKDAELQRI NAELAKYGY

UniProtKB: Ribosomal protein L13a

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Macromolecule #11: 60S ribosomal protein eL13

MacromoleculeName: 60S ribosomal protein eL13 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 26.640346 KDa
SequenceString: MASYASFILR LIHFNLNFVT AFCPLPRAMG RNVAFSNMRR YKCTNKGPNL IKNWFNQPAR KIRRRQTRLQ KAKAIFPCPL QKLRPSVTR CSQKFNLTQR IGRGFTLREL QGVGLSAGKA RQIGIAVDPR RKNKSEESVA RNVARLKEYL SKVTIFSKQA K PEEIQQAI ...String:
MASYASFILR LIHFNLNFVT AFCPLPRAMG RNVAFSNMRR YKCTNKGPNL IKNWFNQPAR KIRRRQTRLQ KAKAIFPCPL QKLRPSVTR CSQKFNLTQR IGRGFTLREL QGVGLSAGKA RQIGIAVDPR RKNKSEESVA RNVARLKEYL SKVTIFSKQA K PEEIQQAI QHQGVVMPVV NPKPVITTGS IAEAKKEVPE NLFMYLRNAR TARNRARLAK RDAEREAAAK TKGGKK

UniProtKB: 60S ribosomal protein L13

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Macromolecule #12: 60S ribosomal protein eL14

MacromoleculeName: 60S ribosomal protein eL14 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 14.785472 KDa
SequenceString:
MSYHRFVEAG RLVYLGDGPD KGKLAVIVDI LSTAAVLIDG PTTGVKRQVV SLRWAMLTDQ VINISEKPTT EQLKKEIEAS PAIDTFMNS VWGKKIQQQR LRAKATFEDM GKIAKLRRER KMAMKRIAMS IK

UniProtKB: Ribosomal protein L14

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Macromolecule #13: 60S ribosomal protein eL15

MacromoleculeName: 60S ribosomal protein eL15 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 24.35373 KDa
SequenceString: MGAYKYVQEL WRKKQSEALH YLFRLRTWEY RQLNAIKRCS HPTRPEKARR LGYKAKRGYV VYRVRVRRGG RKRQCVHGIS YGKPRNIGI VGLKPSRSLR ALAEQKVGRR LPSLRVLNSY WVAQDGSYKW YEVILIDPFH PWIRNDPKIN WICNPVMKRR E CRGLTSAG ...String:
MGAYKYVQEL WRKKQSEALH YLFRLRTWEY RQLNAIKRCS HPTRPEKARR LGYKAKRGYV VYRVRVRRGG RKRQCVHGIS YGKPRNIGI VGLKPSRSLR ALAEQKVGRR LPSLRVLNSY WVAQDGSYKW YEVILIDPFH PWIRNDPKIN WICNPVMKRR E CRGLTSAG RKARGLHNKG IGAMKLRPSK RAAWKRNHTR SLRRYR

UniProtKB: Ribosomal protein L15

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Macromolecule #14: 60S ribosomal protein eL18

MacromoleculeName: 60S ribosomal protein eL18 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 20.207768 KDa
SequenceString:
MGIDLKTGIK KRKVERKEPK SPNPYIRLLH NAFKVVYENT GEDFTKVLTK RLCLSKTNRP VLSVSRLAHL MHKREDKIAV VASTVTNDE RLVTIPRMTI CALKFSATAR SRIEKAGGRC MSFDELLAEK PTGDNCVLLQ GRRSCRAVCK KFGSPGRPNS H VKPKGEHK GRRGARGRHS W

UniProtKB: Ribosomal protein L18

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Macromolecule #15: 60S ribosomal protein eL20

MacromoleculeName: 60S ribosomal protein eL20 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 24.389225 KDa
SequenceString: MDAWTVLLFY QVLKQFQSEF VDALKSRAET RGMMQKYIVY GRRLPSEKFP EPEVFRMTIY APNEIVAKSR FWYHLKSLKK IKRTHGEIL DCQHIEENGD FVKNFGVLLR YRSRVGQHNM YREVRETTSA RAMDKVYSEM AGQCRARYHD IQIINVKEVA D EDVRREKV ...String:
MDAWTVLLFY QVLKQFQSEF VDALKSRAET RGMMQKYIVY GRRLPSEKFP EPEVFRMTIY APNEIVAKSR FWYHLKSLKK IKRTHGEIL DCQHIEENGD FVKNFGVLLR YRSRVGQHNM YREVRETTSA RAMDKVYSEM AGQCRARYHD IQIINVKEVA D EDVRREKV AMFTQKGVRF PHPCPYVHVK RAARTARFQD RAPHVPQ

UniProtKB: LSU ribosomal protein L18AE

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Macromolecule #16: 60S ribosomal protein eL19

MacromoleculeName: 60S ribosomal protein eL19 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 23.199238 KDa
SequenceString: MANLRLQKRL AADVLKCGKR RVRFNPAHEK YIEQASSREA IRSLIESKII AKLDIKGSSR GRIRARHAAK RLGRHSGHGK RRGTREARM PSKTLWIQRQ RVLRRLLSRY RDDEKIDRHL YETLYAKAKG NMFKNKRVLL EHIVGMQKEQ ELEKKKKEEL E QRRKKAEQ ...String:
MANLRLQKRL AADVLKCGKR RVRFNPAHEK YIEQASSREA IRSLIESKII AKLDIKGSSR GRIRARHAAK RLGRHSGHGK RRGTREARM PSKTLWIQRQ RVLRRLLSRY RDDEKIDRHL YETLYAKAKG NMFKNKRVLL EHIVGMQKEQ ELEKKKKEEL E QRRKKAEQ RCKERAEKVA ANRLAFAQTG IFTEKTNK

UniProtKB: Ribosomal protein L19

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Macromolecule #17: 60S ribosomal protein eL21

MacromoleculeName: 60S ribosomal protein eL21 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 18.296393 KDa
SequenceString:
MTHSQGYRCK TRYCFARKFR RHGMANLTTY NRVYKLGQYV NIKVNGAYHK GMPHRYYNGR TGTIFNIGPR ALGVQIQKRV RGRIMLKRI NVRLEHVHPS DCRKNHLERV AANDAARRLA KETGQPVPCL KRQNALPRAA HVVSIASAID ITPLKYDGVF

UniProtKB: Ribosomal protein L21

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Macromolecule #18: 60S ribosomal protein uL14

MacromoleculeName: 60S ribosomal protein uL14 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 15.45004 KDa
SequenceString:
MASKRGRSGG IGNKFRISCG LPVGAVINCA DNSGAKSLNM IAVKNWKGHL NRFPKASVGD MVLITVGRDG KPELRRKVTP AVVVRQRKV WRRKDGVWIY FQDNAGVIIN PKGELKGSSI TGPVAKEAAD RWPKISTTAE CVH

UniProtKB: Ribosomal protein L23

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Macromolecule #19: 60S ribosomal protein uL22

MacromoleculeName: 60S ribosomal protein uL22 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 18.722785 KDa
SequenceString:
MVKYAFKSDN LLVGKSRRDG VKASFKNTYS TCRAIKGMGV KRAFAYLDNV LNHKEIVPFF KFYKGVGRHS QARGSGTDKG RWPEKSVVV VKDILKQAVS NAKHAHKKEE DELYVISYCV NRARGRRRRT FRAHGRISKY ESQPCHVELI VGPKPLDVEK A KALQ

UniProtKB: Ribosomal protein L17

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Macromolecule #20: 60S ribosomal protein uL23

MacromoleculeName: 60S ribosomal protein uL23 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 16.053919 KDa
SequenceString:
MSEKAQALKA QKAILHGTRV EAKKIRTNVH FFRPKTLVHK KAPLYPRNPI KKGSWDDPCD LLRYPIKTDE NTTQIEKNNT IVFIVDRRA SKPEIRKAFE TVFETKVARV NTLITPLGLK KAFIRLAPDV QAMDVASKMG LA

UniProtKB: Ribosomal protein L23A

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Macromolecule #21: 60S ribosomal protein uL24

MacromoleculeName: 60S ribosomal protein uL24 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 15.621398 KDa
SequenceString:
MKLNSAVTAS RRKCRKAYFT ANAETRAKMM SSRLSKELRA EHKIKTMPIR RGDIVEIFTG GHKGTGKVVE VRRRDYKICV EGINQKARN PEAKPVPYPI HPSNCIIKEL YMNGSRYRAI KRRQERNAER LARIGK

UniProtKB: LSU ribosomal protein L24P

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Macromolecule #22: 60S ribosomal protein eL24

MacromoleculeName: 60S ribosomal protein eL24 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 12.005191 KDa
SequenceString:
MVTTNQCSYS GRKILPGYGK RMSRHDKVLL IFLNRKASVH YLNKWNPRKI RWTLASRRAR GKEVIVKARE RVTRKAVVSK RNFLSIDDS KLEALKSKYS RVN

UniProtKB: Ribosomal protein L24A

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Macromolecule #23: 60S ribosomal protein eL27

MacromoleculeName: 60S ribosomal protein eL27 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 15.374895 KDa
SequenceString:
MSQPIIKPGL VCIVLQGRHA GKKAVIVEAL PHNERGSYAI VAGIAALPRR ITNSMSDEEA VCRGRVRSFV KCMNVNHLMP TSCRLENNY KDFISTQTVA DVTKRREIIE TVNKDFQRRI LEHRDTWFFT KLPIDA

UniProtKB: Ribosomal protein L27

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Macromolecule #24: 60S ribosomal protein eL29

MacromoleculeName: 60S ribosomal protein eL29 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 7.363574 KDa
SequenceString:
MAKLKNHTSK NQNRKDHRNG IKKPKKSAYT SHKGMCPKYL RNLRRSRAND PRQSLRPNLN KE

UniProtKB: 60S ribosomal protein L29

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Macromolecule #25: 60S ribosomal protein uL29

MacromoleculeName: 60S ribosomal protein uL29 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 14.316314 KDa
SequenceString:
MPGLKAKDLI GLSQEDLQRK LADLKRELLS LRTMKATAGP VPERIARFRV CKKDVARVLT IINQKARDEA RAAFEGAKYI PKTFRPKLT HAMRCQLTDK QKRLLPNKVM KRKLQFPKLK YAVKV

UniProtKB: LSU ribosomal protein L29P/L35

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Macromolecule #26: 60S ribosomal protein uL30

MacromoleculeName: 60S ribosomal protein uL30 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 27.103406 KDa
SequenceString: MPIPELVLKH RRKVQRDIRS AAERATEKEK RAALVKDHAV KSAQVYARYY IDLVESEKKA RENALKNNQL YVPDEPKVYL VVRIRGIVG VAPKVRTILS LLRLRQINNA VFMRCNKATL NALRIVDHYV TYGEPTVETI RNLIYKRGFA KVNGQRIPIV D NNLIDEQL ...String:
MPIPELVLKH RRKVQRDIRS AAERATEKEK RAALVKDHAV KSAQVYARYY IDLVESEKKA RENALKNNQL YVPDEPKVYL VVRIRGIVG VAPKVRTILS LLRLRQINNA VFMRCNKATL NALRIVDHYV TYGEPTVETI RNLIYKRGFA KVNGQRIPIV D NNLIDEQL SKHNILCAED LVHEIFTCGS SFKEANNFLW PFQLNSAALK DKRNNFAEGG DFGYRGKYIN EFVARMI

UniProtKB: Ribosomal protein L7

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Macromolecule #27: 60S ribosomal protein eL31

MacromoleculeName: 60S ribosomal protein eL31 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 12.184064 KDa
SequenceString:
MGIVYEHTIR LNKAVRGKPS KKAAPIAIRA IRAQVEKLAK VEDVRLDPSV NVFVWSRGIR HVPRRIRLEV RLEGDDEHKF AYVINKDVE NFKHLTTEKK GSEDGSE

UniProtKB: Ribosomal protein L31e

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Macromolecule #28: 60S ribosomal protein eL32

MacromoleculeName: 60S ribosomal protein eL32 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 15.985983 KDa
SequenceString:
MAGEMIHPLP HKIIIKKRKN KFIRFQSERF VRVKPSWRKP RGIDSPMRHQ YRGQRPMVSI GYGSDKETRF MLPCGFFKHR ISTVHELKT LFMNNKTHAA QIAHGVSARS RKQILEEAKK MGVRVLNADA KLRKEEL

UniProtKB: Ribosomal protein L32

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Macromolecule #29: 60S ribosomal protein eL34

MacromoleculeName: 60S ribosomal protein eL34 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 13.528753 KDa
SequenceString:
MADCRVTCRF HSTYRTRSKI VRYKRTPGNR LTSLRVKKLP NAPHCAETGK QIHGIPRVIP QHLTRQQRKV SRPYGGKLCG SALRAHILE SFLNEENQAL REIAAQTAKT AKQTAGKGHA K

UniProtKB: Large ribosomal subunit protein eL34

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Macromolecule #30: 60S ribosomal protein eL33

MacromoleculeName: 60S ribosomal protein eL33 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 13.913122 KDa
SequenceString:
MEEIKGKTFL PAIFVGYQRS RAKQRENVAL VKVSGIYTRA ETDKVLGRLI AIIHSKSGKP IFGKICRPHG NSGMVRARFY TNLPGQLMG HKVRVFYDNI DVGDRATPKY ERLYGNPNAT VTYE

UniProtKB: Ribosomal protein L35a

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Macromolecule #31: 60S ribosomal protein eL36

MacromoleculeName: 60S ribosomal protein eL36 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 10.586708 KDa
SequenceString:
MPGKVFNLKK GGAVVRIVRK KEERKTKPHQ EFVKSIIQEC TGMAPYEMHI IELLRMNKDR HALRYAKKRL GNIKRAKAKR DQLSVYARN I

UniProtKB: Ribosomal protein L36e

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Macromolecule #32: 60S ribosomal protein eL38

MacromoleculeName: 60S ribosomal protein eL38 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 8.605145 KDa
SequenceString:
MPNTITEENL MKMIADGRGK KLVVKSCSKG RLQGKVKLKL RTAKYLYTLT VPNADDAKSI VKGCKDKVEV QYVNDSE

UniProtKB: Ribosomal L38e

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Macromolecule #33: 60S ribosomal protein eL42

MacromoleculeName: 60S ribosomal protein eL42 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 12.311678 KDa
SequenceString:
MVTYPAERRT FCKREGKHTV HKASLYKKGK DSLLAQGKRR YDRKQRGFGG QTKPIFRRKA KTTKKQVIRL TCSSCKCQTF QVLKRCHRL EISRLGATKK SKGSVVF

UniProtKB: Ribosomal protein L44

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Macromolecule #34: 60S ribosomal protein eL22

MacromoleculeName: 60S ribosomal protein eL22 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 19.942402 KDa
SequenceString:
MEGQWILFPD QIVNHLCRHA DHRLQARVSS KSNLKSNYAY YFSPSSEMRP RIKKCVKKEQ GNIRFAVDCS AGKDLELNLN EFADYVRTH FKINNRKHGV AEKVACSVEG DSLVIQTTGY EFAKRYVKYL TKRFLYQDYQ GVFRVLSTDK ETYTLKPYTI E DDDDEAGT DDE

UniProtKB: Large ribosomal subunit protein eL22

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Macromolecule #35: 60S ribosomal protein eL30

MacromoleculeName: 60S ribosomal protein eL30 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 11.698598 KDa
SequenceString:
MSTKVATKKA QDSISLTLTL VVKSGKYTLG TDQAIRNIRD GKAQLVFMAN NCPPLVKSQV DYLCHLSKTP VRVFAGSSRE LGIAVGRQF NVSIMTILEA GDADLTSILG

UniProtKB: Ribosomal protein L30

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Macromolecule #36: 60S ribosomal protein eL37

MacromoleculeName: 60S ribosomal protein eL37 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 11.473345 KDa
SequenceString:
MELVAVKNGN SMSKGTASFG KRHTRIHGSC RRCGKHSFHL QKHECASCGY PSAKMRRYNW SYKSLRRRTQ GTGSMSHMRK VYRAYNSGT LKANARKYLA K

UniProtKB: Ribosomal protein L37

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Macromolecule #37: 60S ribosomal protein eL39

MacromoleculeName: 60S ribosomal protein eL39 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 6.527003 KDa
SequenceString:
MSANKTLERK LRLGKKIRMN KNIPRWVILK DKLKKRWNYK RHHWRRSHLK L

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Macromolecule #38: 60S ribosomal protein eL40

MacromoleculeName: 60S ribosomal protein eL40 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 14.343675 KDa
SequenceString:
MQLIVRSLDR TVALTASPAD SLTSIRQRLL AVYSGHVVDS QRFVFAGRTL DEAKTLGDYS IGESSVLDLV PRLFGGVMEP TLINLAREY NQNMMICRHC YARNPLRATN CRRCRGTNLR KKKLSKYA

UniProtKB: 50S ribosomal protein L40e

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Macromolecule #39: 60S ribosomal protein uL2

MacromoleculeName: 60S ribosomal protein uL2 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 27.090543 KDa
SequenceString: MGRRIRVQRK GAGSVFRAHV ARRLGAAKLR ANDFAERTGS VRGVVKAILH DRGRGAPLAR VQFPNMRGEG RVNELFIAPE GMYTGQEVF SGNKATLHIG NILPVGNIPE GTYVCNVEEK PMDRGCLARA SGTYCLVVAH NMETNKTRIR LPSGQKKLIS S KARAMIGL ...String:
MGRRIRVQRK GAGSVFRAHV ARRLGAAKLR ANDFAERTGS VRGVVKAILH DRGRGAPLAR VQFPNMRGEG RVNELFIAPE GMYTGQEVF SGNKATLHIG NILPVGNIPE GTYVCNVEEK PMDRGCLARA SGTYCLVVAH NMETNKTRIR LPSGQKKLIS S KARAMIGL VAGGGRTDKP LLKAGNAYHK YKAKRNCWPV VRGVAMNPVD HPHGGGNHQH IGVSSCVPRN AVPGQKVGLI AA RRTGCSG GKRR

UniProtKB: Ribosomal protein L8

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Macromolecule #40: 60S ribosomal protein eL41

MacromoleculeName: 60S ribosomal protein eL41 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 2.145636 KDa
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #41: 5S rRNA

MacromoleculeName: 5S rRNA / type: rna / ID: 41 / Number of copies: 1
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 38.672023 KDa
SequenceString:
CAGUUCGGCC AUCCUACGGC GGAAACUCCU UAACUCGUUC CGAAUUAAGA AGAGAAGCGC CGUCAGGCCC CGCCAGUACU GCGAUCGGA GACGUCGUGG GAACACGGGG UGCCGAACUU U

GENBANK: GENBANK: XR_005248657.1

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Macromolecule #42: 5.8S rRNA

MacromoleculeName: 5.8S rRNA / type: rna / ID: 42 / Number of copies: 1
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 45.58627 KDa
SequenceString:
AACGCCCCGC CGGCGGAUGC CUCGGCCCGG GCGGCGACGA AGAGCGCGGC GGAGCGCGAG ACGCGGUGCG GACCCGCCCG CCCCGAGAA GCACCGGCCC UCGAACGCAG CGCGCCCCGG CGCCGCCGCC UCGGUGCCCU GC

GENBANK: GENBANK: KF835560.1

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Macromolecule #43: 28S rRNA

MacromoleculeName: 28S rRNA / type: rna / ID: 43 / Number of copies: 1
Source (natural)Organism: Giardia intestinalis assemblage A (eukaryote)
Molecular weightTheoretical: 871.348812 KDa
SequenceString: CGCGGCCCGA GGCGGCGGGG GCGACGGGCG GAACUUAAGC AUAUCAGUAC GCCCCGGAGG AGAAACCAAC CGGGAUUCCC CGUAGCGGC GAGCGACGCG GGAGGAGCCC GCCCCGAAGG CGCGCUGUGG GGCGCAGGCG CAGGCCCGCC GCGAGGGGGC C CGAGGGCC ...String:
CGCGGCCCGA GGCGGCGGGG GCGACGGGCG GAACUUAAGC AUAUCAGUAC GCCCCGGAGG AGAAACCAAC CGGGAUUCCC CGUAGCGGC GAGCGACGCG GGAGGAGCCC GCCCCGAAGG CGCGCUGUGG GGCGCAGGCG CAGGCCCGCC GCGAGGGGGC C CGAGGGCC CCGCCCGAGA GGGUGCAAGC CCCGUACGGC GGCCGCCGGG CCUGCGCGGC GAGUAGCGCU GCUUGAGCGU GC AGCGCGA AGGGAGGCGC GGCCCUUCCA AGGCUAAAUA CGCCCCGGGA CCGAUAGCGG ACCAAGUAGC GCGAGCGAAC GGU GAAAAG GACGCCCUGC GGCCGCUCAA AAGACCUGAA CCCGGCCGGC CGCCGGCCCG CCGGCCCCGU CUCGAAACAC GGAC CGAGG AGCCACGCGC CGCGGCGAGC CCGAGGGAGC CCCCGCGGCG GAGCGAGCGC GAGACGCCCC GGGCCCGCCA UGCCC CUGC GGGCGUGCGC GGGCCGAGCC GCGGCGCGUG GGCCCGAAAG GCGGUGAUCU AUGCCCGGCG AGGGCGAGGC CGGGCG AAA GCCUGGUGGA GGCCCGCCGC GGUGCUGACG CGCAGAUCGC UCGUCGGAGC CGGGCAUGGG GGCGAAAGAC UCAUCGA AC CGCCUGGUAG CUGGUUGCCU CCGAAAUGUC UCCCAGGACA GCCGCCGCCC CGCAGUUGCG GCCCGUAGAG CGCUGGCC G GCGGGAGCGG GGGGCCUGCC CCUCGCCCGC CCCCCAAACU CCGAAGGGCC GCGCCGCCCC GCCGCUGGCC UGGGCGGGG CGGGCGAAUG CGGGCGGCGC GUGGGCCCCU CCUGGUAAGC AGGACGGGCG AGGCGGGACG AUCCGGACGC CGGGCCAGGG UGCGCCGCC GGGGCCCGCG GAACGGCGUC GGCCGGUCCC GACAGCUGGA AGGUGGCCCC AGAAGUCGGC AUCCUCCAGG G AGUGUGUA ACAACCCACC AGCCGAAUCG GCCGGCCCGG AAAAUGGAGC GCGCCGGAGC CCCGGACCCG CGCCCGGCCG CC GCGCGCG GCGGGUAGGA GGCCGCAGAG GCCCCGGGGG CGAAGGCGGC GCGCAGGCCC CGCCGGACCG GCCUCUGGUG CAG AUCUCG GCAGCAGUAG CCGCUACUCC GCGCCCCGGA GGACUGAGGG GGAGACGGGU UCCGCGGCGC CUGCAUCUGG CCGC GGGUG ACUCGGGCCU AAGCGGCGGG UGAAGACCGG GAAGGGGCGU GCCCGCCCGU CGAACGGGGA GCCGGCGGAG ACUCC GGCA GGCGCGGCCC CCGCGGAGAC GCCCGCCCCC CGGCGACGCG CACGGGGACC GCGGCGGGCG GCGCCCCGGC CCGCGA ACG CCCCGCAGCC CCCGGACGCC UUGCGCGGAG AGGGGGGCCC GGGGGCGGAC CCCGCGCGUC CCCGGCCGCC CCUGAAA AG CCGGGGGGCG CCGGCCGCGC GCCGUACCGA CCGCAGCAGG ACUCCGGGGU CAGCAGCCUC UAGCGCGGGA GCGAACGC G GCUCAGGGAA GUCGGCAAGC CGGCUCCGUA ACCUCGGGAA AAGGAGUGGC UCUGACGGCG CGCCGGGUCA GAACUGGAA CGGACGCGGG GAUCCCGACU GUUUACUAGA AACACAGCGU CGCGAGGGCC GCACCCGGCG CUGGCGCGAC GUGAUUUCUG CCCAGUGCC ACGACCGUCA CCGUGAAGCG AUCCGCCGAA GCCCUGGUAA ACGGCGGGAG UAACUAUGAC UCUCUUAAGG U AGCCAAAU GCCUCGUCGG GCAAUUUCCG ACGUGCAUGA AUGGACCAAC GAGGAUCCCA CUGUCCCGAG CCGCGCCUCC GC GAGCCUC CAGCCUCGGG AACGGGCGAG GGCCGGCCAG CGGGGCAAGA AGACCCUUUU GAGCUUGACU CCAGCCCGGG CCU GUGGGG CGGGGCGGCC GGCGCAGCGC ACAGGGGAGG CCGCGCCCCU GAGACACCCU GACGGCCGCC GCCGCCCCGC UCAC CCGGU CGCGCGGGGA CCCGCCCGGG CGGGGAGUUC GGCUGGGGCG GCGCGCCUGC UACACCGGAC CGCAGGCGUC CCACG GCGG GCUCAGCGAG GACGGAGACC UCCCGCGGAG CAGAAGGGCA CAAGCCCGCC CGACCCGCGC CCCCCGUGCC GGCGCG GGC CGCGAAAGCG GGGCCUACCG AUCCUUCGCC GCCCCGGCCG CGGGCGCGGA GGUGGCAGAA AAGUUACCAC AGGGAUA AC UGGCUUGUGG CCGCCGAGCG CCCGCAGCGA CGCGGCUUUU UGAUCCUUCG AUGUCGGCUC UUCCUACCGU CCGCGCGC A CCGGCGCGGA AGCGUCGGAU UGUUCACCCG UUCAAGGGAU CGUGAGCUGG GUUUAGACCG UCGUGAGACA GGUUAGUUU UACCCUACUG GCCCCGGGGC CAGAGCACGG CGGGCCAGUA CGAGAGGAAC GCCCGCCGCG GGCCGCCAGC CCCGCGGUUG CCCGGCCGG GCAGCGCCGC GCCGCCGCGC CCGGGGGCCC UGCGCUGACC GCCUCUAAGC GCGCACCCCG CCUCGCGCCC C GCCCGGCC GCGCGCCCCA GCCCCGUGCC CCGUCGCCGA GCGGCCCCCG CCCGGGGAGA CCACCCGGCG CGGCGCUCCU GU ACGGCGC AGAGCCCUGC GAUCGCCUGA GGGACGCGCC UGCAGAGCGC

GENBANK: GENBANK: CP110916.1

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Macromolecule #44: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 44 / Number of copies: 27 / Formula: K
Molecular weightTheoretical: 39.098 Da

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Macromolecule #45: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 45 / Number of copies: 129 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #46: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 46 / Number of copies: 2 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #47: SODIUM ION

MacromoleculeName: SODIUM ION / type: ligand / ID: 47 / Number of copies: 33
Molecular weightTheoretical: 22.99 Da

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Macromolecule #48: SPERMIDINE

MacromoleculeName: SPERMIDINE / type: ligand / ID: 48 / Number of copies: 1 / Formula: SPD
Molecular weightTheoretical: 145.246 Da
Chemical component information

ChemComp-SPD:
SPERMIDINE

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Macromolecule #49: water

MacromoleculeName: water / type: ligand / ID: 49 / Number of copies: 3122 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 72.26 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.9000000000000001 µm / Nominal defocus min: 0.5 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

5xy3

Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.49 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 289618
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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About Yorodumi

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News

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Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

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Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

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