8FR8

Structure of Mycobacterium smegmatis Rsh bound to a 70S translation initiation complex

これはPDB形式変換不可エントリーです。

8FR8 の概要

• エントリーDOI: 10.2210/pdb8fr8/pdb
• EMDBエントリー: 29397
• 分子名称: 50S ribosomal protein L33 2, 50S ribosomal protein L2, 50S ribosomal protein L3, ... (58 entities in total)
• 機能のキーワード: starvation sensing, rela/spot homologue, mycobacterium, 70s initiation complex, translation
• 由来する生物種: Mycolicibacterium smegmatis MC2 155; 詳細
• タンパク質・核酸の鎖数: 58
• 化学式量合計: 2345278.06

構造登録者

Majumdar, S.,Sharma, M.R.,Manjari, S.R.,Banavali, N.K.,Agrawal, R.K. (登録日: 2023-01-06, 公開日: 2023-05-17, 最終更新日: 2024-06-19)

主引用文献

Li, Y.,Majumdar, S.,Treen, R.,Sharma, M.R.,Corro, J.,Gamper, H.B.,Manjari, S.R.,Prusa, J.,Banavali, N.K.,Stallings, C.L.,Hou, Y.M.,Agrawal, R.K.,Ojha, A.K.
Starvation sensing by mycobacterial RelA/SpoT homologue through constitutive surveillance of translation.
Proc.Natl.Acad.Sci.USA, 120:e2302006120-e2302006120, 2023

PubMed Abstract: The stringent response, which leads to persistence of nutrient-starved mycobacteria, is induced by activation of the RelA/SpoT homolog (Rsh) upon entry of a deacylated-tRNA in a translating ribosome. However, the mechanism by which Rsh identifies such ribosomes in vivo remains unclear. Here, we show that conditions inducing ribosome hibernation result in loss of intracellular Rsh in a Clp protease-dependent manner. This loss is also observed in nonstarved cells using mutations in Rsh that block its interaction with the ribosome, indicating that Rsh association with the ribosome is important for Rsh stability. The cryo-EM structure of the Rsh-bound 70S ribosome in a translation initiation complex reveals unknown interactions between the ACT domain of Rsh and components of the ribosomal L7/L12 stalk base, suggesting that the aminoacylation status of A-site tRNA is surveilled during the first cycle of elongation. Altogether, we propose a surveillance model of Rsh activation that originates from its constitutive interaction with the ribosomes entering the translation cycle.

PubMed: 37216503
DOI: 10.1073/pnas.2302006120

実験手法

ELECTRON MICROSCOPY (2.76 Å)