8FR5

Crystal structure of the Human Smacovirus 1 Rep domain

Summary for 8FR5

• Entry DOI: 10.2210/pdb8fr5/pdb
• Descriptor: Rep, SODIUM ION, MANGANESE (II) ION, ... (4 entities in total)
• Functional Keywords: dna binding protein, endonuclease, replication, viral protein
• Biological source: Human smacovirus 1
• Total number of polymer chains: 1
• Total formula weight: 11634.85

Authors

Limon, L.K.,Shi, K.,Dao, A.,Rugloski, J.,Tompkins, K.J.,Aihara, H.,Gordon, W.R.,Evans IIII, R.L. (deposition date: 2023-01-06, release date: 2023-12-27)

Primary citation

Limon, L.K.,Shi, K.,Dao, A.,Rugloski, J.,Tompkins, K.J.,Aihara, H.,Gordon, W.R.,Evans 3rd, R.L.
The crystal structure of the human smacovirus 1 Rep domain.
Acta Crystallogr.,Sect.F, 79:295-300, 2023

PubMed Abstract: Replication initiator proteins (Reps) from the HUH endonuclease family process specific single-stranded DNA sequences to initiate rolling-circle replication in viruses. Here, the first crystal structure of the apo state of a Rep domain from the smacovirus family is reported. The structure of the human smacovirus 1 Rep domain was obtained at 1.33 Å resolution and represents an expansion of the HUH endonuclease superfamily, allowing greater diversity in bioconjugation-tag applications.

PubMed: 38051309
DOI: 10.1107/S2053230X23009536

Experimental method

X-RAY DIFFRACTION (1.33 Å)