8FQ4

AAV1 VP3 Only Capsid

This is a non-PDB format compatible entry.

Summary for 8FQ4

• Entry DOI: 10.2210/pdb8fq4/pdb
• EMDB information: 29377
• Descriptor: Capsid protein (1 entity in total)
• Functional Keywords: aav, gene therapy, capsid, vp3, virus like particle
• Biological source: Adeno-associated virus - 1
• Total number of polymer chains: 60
• Total formula weight: 3497965.32

Authors

Mietzsch, M.,McKenna, R. (deposition date: 2023-01-05, release date: 2023-02-08, Last modification date: 2024-06-19)

Primary citation

Mietzsch, M.,Liu, W.,Ma, K.,Bennett, A.,Nelson, A.R.,Gliwa, K.,Chipman, P.,Fu, X.,Bechler, S.,McKenna, R.,Viner, R.
Production and characterization of an AAV1-VP3-only capsid: An analytical benchmark standard.
Mol Ther Methods Clin Dev, 29:460-472, 2023

PubMed Abstract: Adeno-associated viruses (AAVs) are non-enveloped ssDNA icosahedral T = 1 viruses used as vectors for clinical gene delivery. Currently, there are over 200 AAV-related clinical trials and six approved biologics on the market. As such new analytical methods are continually being developed to characterize and monitor the quality and purity of manufactured AAV vectors, these include ion-exchange chromatography and Direct Mass Technology. However, these methods require homogeneous analytical standards with a high molecular weight standard comparable to the mass of an AAV capsid. Described here is the design, production, purification, characterization, and the cryo-electron microscopy structure of an AAV1-VP3-only capsid that fulfills this need as a calibrant to determine capsid mass, charge, homogeneity, and transgene packaging characteristics.

PubMed: 37273903
DOI: 10.1016/j.omtm.2023.05.002

Experimental method

ELECTRON MICROSCOPY (2.27 Å)