8FN4
Cryo-EM structure of RNase-treated RESC-A in trypanosomal RNA editing
Summary for 8FN4
| Entry DOI | 10.2210/pdb8fn4/pdb |
| EMDB information | 29305 |
| Descriptor | RNA-editing substrate-binding complex protein 1 (RESC1), RNA-editing substrate-binding complex protein 2 (RESC2), RNA-editing substrate-binding complex protein 3 (RESC3), ... (6 entities in total) |
| Functional Keywords | heat repeat, trypanosoma, rna editing substrate binding complex, grna, rna binding protein-rna complex, rna binding protein/rna |
| Biological source | Trypanosoma brucei More |
| Total number of polymer chains | 6 |
| Total formula weight | 383422.30 |
| Authors | Liu, S.,Wang, H.,Li, X.,Zhang, F.,Lee, J.K.J.,Li, Z.,Yu, C.,Zhao, X.,Hu, J.J.,Suematsu, T.,Alvarez-Cabrera, A.L.,Liu, Q.,Zhang, L.,Huang, L.,Aphasizheva, I.,Aphasizhev, R.,Zhou, Z.H. (deposition date: 2022-12-26, release date: 2023-07-19, Last modification date: 2024-06-19) |
| Primary citation | Liu, S.,Wang, H.,Li, X.,Zhang, F.,Lee, J.K.J.,Li, Z.,Yu, C.,Hu, J.J.,Zhao, X.,Suematsu, T.,Alvarez-Cabrera, A.L.,Liu, Q.,Zhang, L.,Huang, L.,Aphasizheva, I.,Aphasizhev, R.,Zhou, Z.H. Structural basis of gRNA stabilization and mRNA recognition in trypanosomal RNA editing. Science, 381:eadg4725-eadg4725, 2023 Cited by PubMed Abstract: In , the editosome, composed of RNA-editing substrate-binding complex (RESC) and RNA-editing catalytic complex (RECC), orchestrates guide RNA (gRNA)-programmed editing to recode cryptic mitochondrial transcripts into messenger RNAs (mRNAs). The mechanism of information transfer from gRNA to mRNA is unclear owing to a lack of high-resolution structures for these complexes. With cryo-electron microscopy and functional studies, we have captured gRNA-stabilizing RESC-A and gRNA-mRNA-binding RESC-B and RESC-C particles. RESC-A sequesters gRNA termini, thus promoting hairpin formation and blocking mRNA access. The conversion of RESC-A into RESC-B or -C unfolds gRNA and allows mRNA selection. The ensuing gRNA-mRNA duplex protrudes from RESC-B, likely exposing editing sites to RECC-catalyzed cleavage, uridine insertion or deletion, and ligation. Our work reveals a remodeling event facilitating gRNA-mRNA hybridization and assembly of a macromolecular substrate for the editosome's catalytic modality. PubMed: 37410820DOI: 10.1126/science.adg4725 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.7 Å) |
Structure validation
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