8FHW

Cryo-EM structure of Cryptococcus neoformans trehalose-6-phosphate synthase homotetramer in complex with uridine diphosphate and glucose-6-phosphate

8FHW の概要

• エントリーDOI: 10.2210/pdb8fhw/pdb
• EMDBエントリー: 29172
• 分子名称: Alpha,alpha-trehalose-phosphate synthase (UDP-forming), URIDINE-5'-DIPHOSPHATE, 6-O-phosphono-alpha-D-glucopyranose (3 entities in total)
• 機能のキーワード: glycosyltransferase, complex, transferase
• 由来する生物種: Cryptococcus neoformans var. grubii H99
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 309919.19

構造登録者

Washington, E.J.,Brennan, R.G. (登録日: 2022-12-15, 公開日: 2023-12-20, 最終更新日: 2024-06-26)

主引用文献

Washington, E.J.,Zhou, Y.,Hsu, A.L.,Petrovich, M.,Tenor, J.L.,Toffaletti, D.L.,Guan, Z.,Perfect, J.R.,Borgnia, M.J.,Bartesaghi, A.,Brennan, R.G.
Structures of trehalose-6-phosphate synthase, Tps1, from the fungal pathogen Cryptococcus neoformans : a target for novel antifungals.
Biorxiv, 2024

PubMed Abstract: Invasive fungal diseases are a major threat to human health, resulting in more than 1.5 million annual deaths worldwide. The arsenal of antifungal therapeutics remains limited and is in dire need of novel drugs that target additional biosynthetic pathways that are absent from humans. One such pathway involves the biosynthesis of trehalose. Trehalose is a disaccharide that is required for pathogenic fungi to survive in their human hosts. In the first step of trehalose biosynthesis, trehalose-6-phosphate synthase (Tps1) converts UDP-glucose and glucose-6-phosphate to trehalose-6-phosphate. Here, we report the structures of full-length Tps1 (CnTps1) in unliganded form and in complex with uridine diphosphate and glucose-6-phosphate. Comparison of these two structures reveals significant movement towards the catalytic pocket by the N-terminus upon ligand binding and identifies residues required for substrate-binding, as well as residues that stabilize the tetramer. Intriguingly, an intrinsically disordered domain (IDD), which is conserved amongst Cryptococcal species and closely related Basidiomycetes, extends from each subunit of the tetramer into the "solvent" but is not visible in density maps. We determined that the IDD is not required for Tps1-dependent thermotolerance and osmotic stress survival. Studies with UDP-galactose highlight the exquisite substrate specificity of CnTps1. , these studies expand our knowledge of trehalose biosynthesis in and highlight the potential of developing antifungal therapeutics that disrupt the synthesis of this disaccharide or the formation of a functional tetramer and the use of cryo-EM in the structural characterization of CnTps1-ligand/drug complexes.

PubMed: 36993618
DOI: 10.1101/2023.03.14.530545

実験手法

ELECTRON MICROSCOPY (3.2 Å)