8FFZ

TFIIIA-TFIIIC-Brf1-TBP complex bound to 5S rRNA gene

8FFZ の概要

• エントリーDOI: 10.2210/pdb8ffz/pdb
• EMDBエントリー: 29071
• 分子名称: Transcription factor IIIA, DNA (151-MER), ZINC ION, ... (11 entities in total)
• 機能のキーワード: transcription factor, transcription, transcription-dna complex, transcription/dna
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 758888.90

構造登録者

Talyzina, A.,He, Y. (登録日: 2022-12-11, 公開日: 2023-06-21, 最終更新日: 2023-08-16)

主引用文献

Talyzina, A.,Han, Y.,Banerjee, C.,Fishbain, S.,Reyes, A.,Vafabakhsh, R.,He, Y.
Structural basis of TFIIIC-dependent RNA polymerase III transcription initiation.
Mol.Cell, 83:2641-, 2023

PubMed Abstract: RNA polymerase III (Pol III) is responsible for transcribing 5S ribosomal RNA (5S rRNA), tRNAs, and other short non-coding RNAs. Its recruitment to the 5S rRNA promoter requires transcription factors TFIIIA, TFIIIC, and TFIIIB. Here, we use cryoelectron microscopy (cryo-EM) to visualize the S. cerevisiae complex of TFIIIA and TFIIIC bound to the promoter. Gene-specific factor TFIIIA interacts with DNA and acts as an adaptor for TFIIIC-promoter interactions. We also visualize DNA binding of TFIIIB subunits, Brf1 and TBP (TATA-box binding protein), which results in the full-length 5S rRNA gene wrapping around the complex. Our smFRET study reveals that the DNA within the complex undergoes both sharp bending and partial dissociation on a slow timescale, consistent with the model predicted from our cryo-EM results. Our findings provide new insights into the transcription initiation complex assembly on the 5S rRNA promoter and allow us to directly compare Pol III and Pol II transcription adaptations.

PubMed: 37402369
DOI: 10.1016/j.molcel.2023.06.015

実験手法

ELECTRON MICROSCOPY (3.8 Å)