8FFI

Structure of tetramerized MapSPARTA upon guide RNA-mediated target DNA binding

8FFI の概要

• エントリーDOI: 10.2210/pdb8ffi/pdb
• EMDBエントリー: 29043 29219 29222 29223 29224 29225 29226
• 分子名称: TIR-APAZ, short pAgo, guide RNA, ... (5 entities in total)
• 機能のキーワード: pago, sparta, argonuare, oligomerization, tir domain, nad, immune system
• 由来する生物種: Maribacter polysiphoniae; 詳細
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 502853.03

構造登録者

Shen, Z.F.,Yang, X.Y.,Fu, T.M. (登録日: 2022-12-08, 公開日: 2023-08-23, 最終更新日: 2023-09-20)

主引用文献

Shen, Z.,Yang, X.Y.,Xia, S.,Huang, W.,Taylor, D.J.,Nakanishi, K.,Fu, T.M.
Oligomerization-mediated activation of a short prokaryotic Argonaute.
Nature, 621:154-161, 2023

PubMed Abstract: Although eukaryotic and long prokaryotic Argonaute proteins (pAgos) cleave nucleic acids, some short pAgos lack nuclease activity and hydrolyse NAD(P) to induce bacterial cell death. Here we present a hierarchical activation pathway for SPARTA, a short pAgo consisting of an Argonaute (Ago) protein and TIR-APAZ, an associated protein. SPARTA progresses through distinct oligomeric forms, including a monomeric apo state, a monomeric RNA-DNA-bound state, two dimeric RNA-DNA-bound states and a tetrameric RNA-DNA-bound active state. These snapshots together identify oligomerization as a mechanistic principle of SPARTA activation. The RNA-DNA-binding channel of apo inactive SPARTA is occupied by an auto-inhibitory motif in TIR-APAZ. After the binding of RNA-DNA, SPARTA transitions from a monomer to a symmetric dimer and then an asymmetric dimer, in which two TIR domains interact through charge and shape complementarity. Next, two dimers assemble into a tetramer with a central TIR cluster responsible for hydrolysing NAD(P). In addition, we observe unique features of interactions between SPARTA and RNA-DNA, including competition between the DNA 3' end and the auto-inhibitory motif, interactions between the RNA G2 nucleotide and Ago, and splaying of the RNA-DNA duplex by two loops exclusive to short pAgos. Together, our findings provide a mechanistic basis for the activation of short pAgos, a large section of the Ago superfamily.

PubMed: 37494956
DOI: 10.1038/s41586-023-06456-z

実験手法

ELECTRON MICROSCOPY (2.7 Å)