8FC4

Crystal structure of the A2058-N6-dimethylated Thermus thermophilus 70S ribosome in complex with protein Y, hygromycin A, and erythromycin at 2.45A resolution

This is a non-PDB format compatible entry.

Summary for 8FC4

• Entry DOI: 10.2210/pdb8fc4/pdb
• Descriptor: 23S Ribosomal RNA, 50S ribosomal protein L14, 50S ribosomal protein L15, ... (61 entities in total)
• Functional Keywords: hygromycin a; macrolides; ketolides; erythromycin; azithromycin; telithromycin; antibiotic; synergy; erm methyltransferase, x-ray structure; 70s ribosome; peptidyl transferase center; nascent peptide exit tunnel, ribosome
• Biological source: Escherichia coli K-12; More
• Total number of polymer chains: 106
• Total formula weight: 4426447.13

Authors

Chen, C.-W.,Syroegin, E.A.,Svetlov, M.S.,Polikanov, Y.S. (deposition date: 2022-12-01, release date: 2023-07-26)

• Primary citation: Chen, C.W.,Leimer, N.,Syroegin, E.A.,Dunand, C.,Bulman, Z.P.,Lewis, K.,Polikanov, Y.S.,Svetlov, M.S.; Structural insights into the mechanism of overcoming Erm-mediated resistance by macrolides acting together with hygromycin-A.; Nat Commun, 14:4196-4196, 2023; PubMed: 37452045; DOI: 10.1038/s41467-023-39653-5

Experimental method

X-RAY DIFFRACTION (2.45 Å)