8F7M

Crystal Structure of HLA-B*57:01-TW10-T242N complex

Summary for 8F7M

• Entry DOI: 10.2210/pdb8f7m/pdb
• Descriptor: heavy chain HLA-B*57:01, Beta-2-microglobulin, T242N peptide (TW10 mutant), ... (6 entities in total)
• Functional Keywords: tcr, t cell, hla-b*57:01, tw10, t242n, gag, hiv, hiv controllers, immune system
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 3
• Total formula weight: 45057.94

Authors

Chatzileontiadou, D.S.M.,Lobos, C.A.,Gras, S. (deposition date: 2022-11-18, release date: 2024-06-05, Last modification date: 2024-12-18)

Primary citation

Chatzileontiadou, D.S.M.,Lobos, C.A.,Robson, H.,Almedia, C.A.,Szeto, C.,Castley, A.,D'Orsogna, L.J.,Gras, S.
Public T cell clonotypes are selected in HLA-B ∗ 57:01 + /HIV + patients independently of the viral load.
Cell Rep, 43:114555-114555, 2024

PubMed Abstract: HIV controllers can control viral replication and remain healthy, but the mechanism behind this control is unknown. Despite human leukocyte antigen (HLA) diversity in the population, almost 50% of HIV controllers express the HLA-B57:01 molecule, which presents, among others, the Gag-derived epitope TW10. Given TW10's presentation in early infection, TW10-specific T cells could participate in the control of HIV. Here, we study the strength and functionality of TW10-specific T cells from HLA-B57:01/HIV controller and non-controller individuals. We determine the TW10-specific T cell receptor (TCR) repertoire, revealing a bias in TCR gene usage with the presence of a public TCR. We determine that the T cell response is polyfunctional regardless of the viral load, despite the low affinity of TW10-specific TCRs. We solve the crystal structure of HLA-B57:01-TW10 in complex with a TCR, providing the basis of recognition that underpins the strong TRBV5 bias observed in TW10-specific clonotypes.

PubMed: 39083376
DOI: 10.1016/j.celrep.2024.114555

Experimental method

X-RAY DIFFRACTION (1.88 Å)