8F1E

Cryo-EM structure of Kap114 bound to Gsp1 (RanGTP) and H2A-H2B

This is a non-PDB format compatible entry.

Summary for 8F1E

• Entry DOI: 10.2210/pdb8f1e/pdb
• EMDB information: 28782 28788 28796
• Descriptor: Importin subunit beta-5, Histone H2A.2, Histone H2B.2, ... (6 entities in total)
• Functional Keywords: karyopherin beta, nuclear transport, gtpase, histone chaperone, histones, protein transport-structural protein-nuclear protein complex, protein transport/structural protein/nuclear protein
• Biological source: Saccharomyces cerevisiae S288C; More
• Total number of polymer chains: 4
• Total formula weight: 162999.89

Authors

Jiou, J.,Chook, Y.M. (deposition date: 2022-11-04, release date: 2023-07-26)

• Primary citation: Jiou, J.,Shaffer, J.M.,Bernades, N.E.,Fung, H.Y.J.,Kikumoto Dias, J.,D'Arcy, S.,Chook, Y.M.; Mechanism of RanGTP priming H2A-H2B release from Kap114 in an atypical RanGTP•Kap114•H2A-H2B complex.; Proc.Natl.Acad.Sci.USA, 120:e2301199120-e2301199120, 2023; PubMed: 37450495; DOI: 10.1073/pnas.2301199120

Experimental method

ELECTRON MICROSCOPY (3.28 Å)