8F0H

Structure of SARS-CoV-2 spike with antibody Fabs 2A10 and 1H2 (Local refinement of the RBD and Fabs 1H2 and 2A10)

8F0H の概要

• エントリーDOI: 10.2210/pdb8f0h/pdb
• EMDBエントリー: 28757
• 分子名称: Antibody Fab 1H2 heavy chain, Spike glycoprotein, Antibody Fab 1H2 light chain, ... (6 entities in total)
• 機能のキーワード: viral protein, glycoprotein, immune system, antibody, sars-cov-2, covid, viral protein-immune system complex, coronavirus, viral protein/immune system
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 183886.92

構造登録者

Yu, X.,Zyla, D.,Hastie, K.M.,Saphire, E.O. (登録日: 2022-11-02, 公開日: 2023-05-03, 最終更新日: 2024-11-20)

主引用文献

Hastie, K.M.,Yu, X.,Ana-Sosa-Batiz, F.,Zyla, D.S.,Harkins, S.S.,Hariharan, C.,Wasserman, H.,Zandonatti, M.A.,Miller, R.,Maule, E.,Kim, K.,Valentine, K.M.,Shresta, S.,Saphire, E.O.
Potent Omicron-neutralizing antibodies isolated from a patient vaccinated 6 months before Omicron emergence.
Cell Rep, 42:112421-112421, 2023

PubMed Abstract: Therapeutic antibodies are an important tool in the arsenal against coronavirus infection. However, most antibodies developed early in the pandemic have lost most or all efficacy against newly emergent strains of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), particularly those of the Omicron lineage. Here, we report the identification of a panel of vaccinee-derived antibodies that have broad-spectrum neutralization activity. Structural and biochemical characterization of the three broadest-spectrum antibodies reveal complementary footprints and differing requirements for avidity to overcome variant-associated mutations in their binding footprints. In the K18 mouse model of infection, these three antibodies exhibit protective efficacy against BA.1 and BA.2 infection. This study highlights the resilience and vulnerabilities of SARS-CoV-2 antibodies and provides road maps for further development of broad-spectrum therapeutics.

PubMed: 37083327
DOI: 10.1016/j.celrep.2023.112421

実験手法

ELECTRON MICROSCOPY (3.18 Å)