8F04

Structure of elevenin-Vc1 from venom of the Australian cone snail Conus victoriae

Summary for 8F04

• Entry DOI: 10.2210/pdb8f04/pdb
• NMR Information: BMRB: 31054
• Descriptor: Elevenin-Vc1 (1 entity in total)
• Functional Keywords: venom, hormone-like, hormone
• Biological source: Conus victoriae
• Total number of polymer chains: 1
• Total formula weight: 2125.61

Authors

Krishnarjuna, B.,Sunanda, P.,Norton, R.S. (deposition date: 2022-11-01, release date: 2023-09-13, Last modification date: 2024-11-06)

Primary citation

Krishnarjuna, B.,Sunanda, P.,Seow, J.,Tae, H.S.,Robinson, S.D.,Belgi, A.,Robinson, A.J.,Safavi-Hemami, H.,Adams, D.J.,Norton, R.S.
Characterisation of Elevenin-Vc1 from the Venom of Conus victoriae : A Structural Analogue of alpha-Conotoxins.
Mar Drugs, 21:-, 2023

PubMed Abstract: Elevenins are peptides found in a range of organisms, including arthropods, annelids, nematodes, and molluscs. They consist of 17 to 19 amino acid residues with a single conserved disulfide bond. The subject of this study, elevenin-Vc1, was first identified in the venom of the cone snail ( , , 11-18). Although numerous elevenin sequences have been reported, their physiological function is unclear, and no structural information is available. Upon intracranial injection in mice, elevenin-Vc1 induced hyperactivity at doses of 5 or 10 nmol. The structure of elevenin-Vc1, determined using nuclear magnetic resonance spectroscopy, consists of a short helix and a bend region stabilised by the single disulfide bond. The elevenin-Vc1 structural fold is similar to that of α-conotoxins such as α-RgIA and α-ImI, which are also found in the venoms of cone snails and are antagonists at specific subtypes of nicotinic acetylcholine receptors (nAChRs). In an attempt to mimic the functional motif, Asp-Pro-Arg, of α-RgIA and α-ImI, we synthesised an analogue, designated elevenin-Vc1-DPR. However, neither elevenin-Vc1 nor the analogue was active at six different human nAChR subtypes (α1β1εδ, α3β2, α3β4, α4β2, α7, and α9α10) at 1 µM concentrations.

PubMed: 36827123
DOI: 10.3390/md21020081

Experimental method

SOLUTION NMR