8EUS

Crystal structure of NPC1 luminal domain C

8EUS の概要

• エントリーDOI: 10.2210/pdb8eus/pdb
• 分子名称: NPC intracellular cholesterol transporter 1 (2 entities in total)
• 機能のキーワード: niemann pick, luminal domain, cholesterol, ebola, virus entry, membrane protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 60334.91

構造登録者

Odongo, L.,Pornillos, O. (登録日: 2022-10-19, 公開日: 2023-02-22, 最終更新日: 2024-10-23)

主引用文献

Odongo, L.,Zadrozny, K.K.,Diehl, W.E.,Luban, J.,White, J.M.,Ganser-Pornillos, B.K.,Tamm, L.K.,Pornillos, O.
Purification and structure of luminal domain C of human Niemann-Pick C1 protein.
Acta Crystallogr.,Sect.F, 79:45-50, 2023

PubMed Abstract: Niemann-Pick C1 protein (NPC1) is a membrane protein that primarily resides in late endosomes and lysosomes, and plays an important role in cholesterol homeostasis in the cell. The second luminal domain of NPC1 (NPC1-C) serves as the intracellular receptor for Ebola and Marburg viruses. Here, the recombinant production of nonglycosylated and glycosylated NPC1-C and a new crystal form of the nonglycosylated protein are reported. The crystals belonged to space group P2 and diffracted to 2.3 Å resolution. The structure is similar to other reported structures of NPC1-C, with differences observed in the protruding loops when compared with NPC1-C in complex with Ebola virus glycoprotein or NPC2.

PubMed: 36748341
DOI: 10.1107/S2053230X23000705

実験手法

X-RAY DIFFRACTION (2.3 Å)