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8EOR

Liver carboxylesterase 1

Summary for 8EOR
Entry DOI10.2210/pdb8eor/pdb
EMDB information23427 28465
DescriptorLiver carboxylesterase 1, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ETHYL ACETATE (3 entities in total)
Functional Keywordscarboxylesterase, human liver, hydrolase
Biological sourceHomo sapiens (human)
Total number of polymer chains3
Total formula weight189787.26
Authors
Zhang, Z.,Yu, E. (deposition date: 2022-10-04, release date: 2023-05-03, Last modification date: 2023-11-15)
Primary citationSu, C.C.,Lyu, M.,Zhang, Z.,Miyagi, M.,Huang, W.,Taylor, D.J.,Yu, E.W.
High-resolution structural-omics of human liver enzymes.
Cell Rep, 42:112609-112609, 2023
Cited by
PubMed Abstract: We applied raw human liver microsome lysate to a holey carbon grid and used cryo-electron microscopy (cryo-EM) to define its composition. From this sample we identified and simultaneously determined high-resolution structural information for ten unique human liver enzymes involved in diverse cellular processes. Notably, we determined the structure of the endoplasmic bifunctional protein H6PD, where the N- and C-terminal domains independently possess glucose-6-phosphate dehydrogenase and 6-phosphogluconolactonase enzymatic activity, respectively. We also obtained the structure of heterodimeric human GANAB, an ER glycoprotein quality-control machinery that contains a catalytic α subunit and a noncatalytic β subunit. In addition, we observed a decameric peroxidase, PRDX4, which directly contacts a disulfide isomerase-related protein, ERp46. Structural data suggest that several glycosylations, bound endogenous compounds, and ions associate with these human liver enzymes. These results highlight the importance of cryo-EM in facilitating the elucidation of human organ proteomics at the atomic level.
PubMed: 37289586
DOI: 10.1016/j.celrep.2023.112609
PDB entries with the same primary citation
Experimental method
ELECTRON MICROSCOPY (2.67 Å)
Structure validation

226707

건을2024-10-30부터공개중

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