8ENV
In situ cryo-EM structure of Pseudomonas phage E217 tail baseplate in C6 map
Summary for 8ENV
| Entry DOI | 10.2210/pdb8env/pdb |
| EMDB information | 28280 |
| Descriptor | Sheath protein gp31, Structural protein gp33, Sheath initiator gp34, ... (6 entities in total) |
| Functional Keywords | pseudomonas, phage, baseplate, virus |
| Biological source | Pseudomonas phage vB_PaeM_E217 More |
| Total number of polymer chains | 36 |
| Total formula weight | 1142790.43 |
| Authors | Li, F.,Cingolani, G.,Hou, C. (deposition date: 2022-09-30, release date: 2023-07-26, Last modification date: 2024-06-19) |
| Primary citation | Li, F.,Hou, C.D.,Lokareddy, R.K.,Yang, R.,Forti, F.,Briani, F.,Cingolani, G. High-resolution cryo-EM structure of the Pseudomonas bacteriophage E217. Nat Commun, 14:4052-4052, 2023 Cited by PubMed Abstract: E217 is a Pseudomonas phage used in an experimental cocktail to eradicate cystic fibrosis-associated Pseudomonas aeruginosa. Here, we describe the structure of the whole E217 virion before and after DNA ejection at 3.1 Å and 4.5 Å resolution, respectively, determined using cryogenic electron microscopy (cryo-EM). We identify and build de novo structures for 19 unique E217 gene products, resolve the tail genome-ejection machine in both extended and contracted states, and decipher the complete architecture of the baseplate formed by 66 polypeptide chains. We also determine that E217 recognizes the host O-antigen as a receptor, and we resolve the N-terminal portion of the O-antigen-binding tail fiber. We propose that E217 design principles presented in this paper are conserved across PB1-like Myoviridae phages of the Pbunavirus genus that encode a ~1.4 MDa baseplate, dramatically smaller than the coliphage T4. PubMed: 37422479DOI: 10.1038/s41467-023-39756-z PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.42 Å) |
Structure validation
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