8EKG

MHETase variant Thr159Val, Met192Tyr, Tyr252Phe, Tyr503Trp

8EKG の概要

• エントリーDOI: 10.2210/pdb8ekg/pdb
• 分子名称: Mono(2-hydroxyethyl) terephthalate hydrolase, CALCIUM ION, DI(HYDROXYETHYL)ETHER, ... (6 entities in total)
• 機能のキーワード: protein engineering, mhetase, plastic degradation, hydrolase
• 由来する生物種: Ideonella sakaiensis
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 375648.07

構造登録者

Saunders, J.W.,Frkic, R.L.,Jackson, C.J. (登録日: 2022-09-20, 公開日: 2023-10-18, 最終更新日: 2024-07-10)

• 主引用文献: Saunders, J.W.,Damry, A.M.,Vongsouthi, V.,Spence, M.A.,Frkic, R.L.,Gomez, C.,Yates, P.A.,Matthews, D.S.,Tokuriki, N.,McLeod, M.D.,Jackson, C.J.; Increasing the Soluble Expression and Whole-Cell Activity of the Plastic-Degrading Enzyme MHETase through Consensus Design.; Biochemistry, 63:1663-1673, 2024; PubMed: 38885634; DOI: 10.1021/acs.biochem.4c00165

実験手法

X-RAY DIFFRACTION (2.65 Å)