8EFG

Crystal structure of human TATDN1 bound to dAMP and two zinc ions

8EFG の概要

• エントリーDOI: 10.2210/pdb8efg/pdb
• 分子名称: Deoxyribonuclease TATDN1, ZINC ION, SODIUM ION, ... (8 entities in total)
• 機能のキーワード: nuclease, tatd, ap-endonuclease, exonuclease, hydrolase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34975.33

構造登録者

Dorival, J.,Eichman, B.F. (登録日: 2022-09-08, 公開日: 2023-02-22, 最終更新日: 2024-11-13)

主引用文献

Dorival, J.,Eichman, B.F.
Human and bacterial TatD enzymes exhibit apurinic/apyrimidinic (AP) endonuclease activity.
Nucleic Acids Res., 51:2838-2849, 2023

PubMed Abstract: TatD enzymes are evolutionarily conserved deoxyribonucleases associated with DNA repair, apoptosis, development, and parasite virulence. Three TatD paralogs exist in humans, but their nuclease functions are unknown. Here, we describe the nuclease activities of two of the three human TatD paralogs, TATDN1 and TATDN3, which represent two phylogenetically distinct clades based on unique active site motifs. We found that in addition to 3'-5' exonuclease activity associated with other TatD proteins, both TATDN1 and TATDN3 exhibited apurinic/apyrimidinic (AP) endonuclease activity. The AP endonuclease activity was observed only in double-stranded DNA, whereas exonuclease activity was operative primarily in single-stranded DNA. Both nuclease activities were observed in the presence of Mg2+ or Mn2+, and we identified several divalent metal cofactors that inhibited exonuclease and supported AP endonuclease activity. Biochemical analysis and a crystal structure of TATDN1 bound to 2'-deoxyadenosine 5'-monophosphate in the active site are consistent with two-metal ion catalysis, and we identify several residues that differentiate nuclease activities in the two proteins. In addition, we show that the three Escherichia coli TatD paralogs are also AP endonucleases, indicating that this activity is conserved across evolution. Together, these results indicate that TatD enzymes constitute a family of ancient AP endonucleases.

PubMed: 36881763
DOI: 10.1093/nar/gkad133

実験手法

X-RAY DIFFRACTION (1.5 Å)