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8DQV

The 1.52 angstrom CryoEM structure of the [NiFe]-hydrogenase Huc from Mycobacterium smegmatis - catalytic dimer (Huc2S2L)

Summary for 8DQV
Entry DOI10.2210/pdb8dqv/pdb
Related7UTD 7UUR 7UUS
EMDB information26767 26801 26802 27661
DescriptorHydrogenase-2, large subunit, Hydrogenase-2, small subunit, OXYGEN ATOM, ... (9 entities in total)
Functional Keywords[nife] hydrogenase, membrane-associated, complex, quinone transport, electron transport
Biological sourceMycolicibacterium smegmatis
More
Total number of polymer chains4
Total formula weight187167.04
Authors
Grinter, R.,Venugopal, H.,Kropp, A.,Greening, C. (deposition date: 2022-07-20, release date: 2023-01-04, Last modification date: 2024-11-13)
Primary citationGrinter, R.,Kropp, A.,Venugopal, H.,Senger, M.,Badley, J.,Cabotaje, P.R.,Jia, R.,Duan, Z.,Huang, P.,Stripp, S.T.,Barlow, C.K.,Belousoff, M.,Shafaat, H.S.,Cook, G.M.,Schittenhelm, R.B.,Vincent, K.A.,Khalid, S.,Berggren, G.,Greening, C.
Structural basis for bacterial energy extraction from atmospheric hydrogen.
Nature, 615:541-547, 2023
Cited by
PubMed Abstract: Diverse aerobic bacteria use atmospheric H as an energy source for growth and survival. This globally significant process regulates the composition of the atmosphere, enhances soil biodiversity and drives primary production in extreme environments. Atmospheric H oxidation is attributed to uncharacterized members of the [NiFe] hydrogenase superfamily. However, it remains unresolved how these enzymes overcome the extraordinary catalytic challenge of oxidizing picomolar levels of H amid ambient levels of the catalytic poison O and how the derived electrons are transferred to the respiratory chain. Here we determined the cryo-electron microscopy structure of the Mycobacterium smegmatis hydrogenase Huc and investigated its mechanism. Huc is a highly efficient oxygen-insensitive enzyme that couples oxidation of atmospheric H to the hydrogenation of the respiratory electron carrier menaquinone. Huc uses narrow hydrophobic gas channels to selectively bind atmospheric H at the expense of O, and 3 [3Fe-4S] clusters modulate the properties of the enzyme so that atmospheric H oxidation is energetically feasible. The Huc catalytic subunits form an octameric 833 kDa complex around a membrane-associated stalk, which transports and reduces menaquinone 94 Å from the membrane. These findings provide a mechanistic basis for the biogeochemically and ecologically important process of atmospheric H oxidation, uncover a mode of energy coupling dependent on long-range quinone transport, and pave the way for the development of catalysts that oxidize H in ambient air.
PubMed: 36890228
DOI: 10.1038/s41586-023-05781-7
PDB entries with the same primary citation
Experimental method
ELECTRON MICROSCOPY (1.52 Å)
Structure validation

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