8DL6

Cryo-EM structure of human ferroportin/slc40 bound to Ca2+ in nanodisc

Summary for 8DL6

• Entry DOI: 10.2210/pdb8dl6/pdb
• EMDB information: 27497
• Descriptor: Solute carrier family 40 member 1, 11F9 light-chain, 11F9 heavy-chain, ... (5 entities in total)
• Functional Keywords: slc40, fpn, ferroportin, iron transporter, calcium, ca2+, human, nanodisc, membrane protein
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 3
• Total formula weight: 112735.62

Authors

Shen, J.,Wilbon, A.S.,Pan, Y.,Zhou, M. (deposition date: 2022-07-07, release date: 2023-01-25, Last modification date: 2025-05-28)

Primary citation

Shen, J.,Wilbon, A.S.,Zhou, M.,Pan, Y.
Mechanism of Ca 2+ transport by ferroportin.
Elife, 12:-, 2023

PubMed Abstract: Ferroportin (Fpn) is a transporter that releases ferrous ion (Fe) from cells and is important for homeostasis of iron in circulation. Export of one Fe by Fpn is coupled to import of two H to maintain charge balance. Here, we show that human Fpn (HsFpn) binds to and mediates Ca transport. We determine the structure of Ca-bound HsFpn and identify a single Ca binding site distinct from the Fe binding sites. Further studies validate the Ca binding site and show that Ca transport is not coupled to transport of another ion. In addition, Ca transport is significantly inhibited in the presence of Fe but not vice versa. Function of Fpn as a Ca uniporter may allow regulation of iron homeostasis by Ca.

PubMed: 36648329
DOI: 10.7554/eLife.82947

Experimental method

ELECTRON MICROSCOPY (3 Å)