8DKC

P. gingivalis RNA Polymerase

8DKC の概要

• エントリーDOI: 10.2210/pdb8dkc/pdb
• EMDBエントリー: 27487
• 分子名称: DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... (4 entities in total)
• 機能のキーワード: porphyromonas gingivalis, rna polymerase, transcription, cfb group bacteria
• 由来する生物種: Porphyromonas gingivalis; 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 390445.01

構造登録者

Liu, B.,Bu, F. (登録日: 2022-07-05, 公開日: 2023-06-21, 最終更新日: 2024-11-06)

主引用文献

Bu, F.,Wang, X.,Li, M.,Ma, L.,Wang, C.,Hu, Y.,Cao, Z.,Liu, B.
Cryo-EM Structure of Porphyromonas gingivalis RNA Polymerase.
J.Mol.Biol., 436:168568-168568, 2024

PubMed Abstract: Porphyromonas gingivalis, an anaerobic CFB (Cytophaga, Fusobacterium, and Bacteroides) group bacterium, is the keystone pathogen of periodontitis and has been implicated in various systemic diseases. Increased antibiotic resistance and lack of effective antibiotics necessitate a search for new intervention strategies. Here we report a 3.5 Å resolution cryo-EM structure of P. gingivalis RNA polymerase (RNAP). The structure displays new structural features in its ω subunit and multiple domains in β and β' subunits, which differ from their counterparts in other bacterial RNAPs. Superimpositions with E. coli RNAP holoenzyme and initiation complex further suggest that its ω subunit may contact the σ4 domain, thereby possibly contributing to the assembly and stabilization of initiation complexes. In addition to revealing the unique features of P. gingivalis RNAP, our work offers a framework for future studies of transcription regulation in this important pathogen, as well as for structure-based drug development.

PubMed: 38583515
DOI: 10.1016/j.jmb.2024.168568

実験手法

ELECTRON MICROSCOPY (3.5 Å)