8DH3

T7 RNA polymerase elongation complex with unnatural base dPa

8DH3 の概要

• エントリーDOI: 10.2210/pdb8dh3/pdb
• 分子名称: T7 RNA polymerase, Template strand DNA, RNA, ... (4 entities in total)
• 機能のキーワード: t7 rna polymerase, elongation complex, unnatural base, ds-pa, synthetic dna, transcription, transferase-dna-rna complex, transferase/dna/rna
• 由来する生物種: Escherichia phage T7; 詳細
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 444051.70

構造登録者

Oh, J.,Wang, D. (登録日: 2022-06-24, 公開日: 2023-02-01, 最終更新日: 2023-10-25)

主引用文献

Oh, J.,Kimoto, M.,Xu, H.,Chong, J.,Hirao, I.,Wang, D.
Structural basis of transcription recognition of a hydrophobic unnatural base pair by T7 RNA polymerase.
Nat Commun, 14:195-195, 2023

PubMed Abstract: Bacteriophage T7 RNA polymerase (T7 RNAP) is widely used for synthesizing RNA molecules with synthetic modifications and unnatural base pairs (UBPs) for a variety of biotechnical and therapeutic applications. However, the molecular basis of transcription recognition of UBPs by T7 RNAP remains poorly understood. Here we focused on a representative UBP, 7-(2-thienyl)-imidazo[4,5-b]pyridine (Ds) and pyrrole 2-carbaldehyde (Pa), and investigated how the hydrophobic Ds-Pa pair is recognized by T7 RNAP. Our kinetic assays revealed that T7 RNAP selectively recognizes the Ds or Pa base in the templates and preferentially incorporates their cognate unnatural base nucleotide substrate (PaTP or DsTP) over natural NTPs. Our structural studies reveal that T7 RNAP recognizes the unnatural substrates at the pre-insertion state in a distinct manner compared to natural substrates. These results provide mechanistic insights into transcription recognition of UBP by T7 RNAP and provide valuable information for designing the next generation of UBPs.

PubMed: 36635281
DOI: 10.1038/s41467-022-35755-8

実験手法

X-RAY DIFFRACTION (3 Å)