8D6M

Nanorana parkeri saxiphilin:STX (co-crystal)

8D6M の概要

• エントリーDOI: 10.2210/pdb8d6m/pdb
• 分子名称: Saxiphilin, PENTAETHYLENE GLYCOL, [(3aS,4R,10aS)-2,6-diamino-10,10-dihydroxy-3a,4,9,10-tetrahydro-3H,8H-pyrrolo[1,2-c]purin-4-yl]methyl carbamate, ... (4 entities in total)
• 機能のキーワード: saxiphilin, toxin resistance, saxitoxin, antitoxin
• 由来する生物種: Nanorana parkeri
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 95241.21

構造登録者

Zakrzewska, S.,Chen, Z.,Minor, D.L. (登録日: 2022-06-06, 公開日: 2022-11-02, 最終更新日: 2024-11-06)

主引用文献

Chen, Z.,Zakrzewska, S.,Hajare, H.S.,Alvarez-Buylla, A.,Abderemane-Ali, F.,Bogan, M.,Ramirez, D.,O'Connell, L.A.,Du Bois, J.,Minor Jr., D.L.
Definition of a saxitoxin (STX) binding code enables discovery and characterization of the anuran saxiphilin family.
Proc.Natl.Acad.Sci.USA, 119:e2210114119-e2210114119, 2022

PubMed Abstract: American bullfrog () saxiphilin (Sxph) is a high-affinity "toxin sponge" protein thought to prevent intoxication by saxitoxin (STX), a lethal bis-guanidinium neurotoxin that causes paralytic shellfish poisoning (PSP) by blocking voltage-gated sodium channels (Nas). How specific Sxph interactions contribute to STX binding has not been defined and whether other organisms have similar proteins is unclear. Here, we use mutagenesis, ligand binding, and structural studies to define the energetic basis of Sxph:STX recognition. The resultant STX "recognition code" enabled engineering of Sxph to improve its ability to rescue Nas from STX and facilitated discovery of 10 new frog and toad Sxphs. Definition of the STX binding code and Sxph family expansion among diverse anurans separated by ∼140 My of evolution provides a molecular basis for understanding the roles of toxin sponge proteins in toxin resistance and for developing novel proteins to sense or neutralize STX and related PSP toxins.

PubMed: 36279441
DOI: 10.1073/pnas.2210114119

実験手法

X-RAY DIFFRACTION (2 Å)