8D65

ELIC apo in 2:1:1 POPC:POPE:POPG nanodisc

Summary for 8D65

• Entry DOI: 10.2210/pdb8d65/pdb
• Related: 8D63 8D64 8D66 8D67 8D68
• EMDB information: 27215 27216 27217 27218 27219 27220
• Descriptor: Erwinia ligand-gated ion channel, (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate (2 entities in total)
• Functional Keywords: elic, ion channel, plgic, structural protein
• Biological source: Dickeya dadantii
• Total number of polymer chains: 5
• Total formula weight: 188140.04

Authors

Petroff II, J.T.,Deng, Z.,Rau, M.J.,Fitzpatrick, J.A.J.,Yuan, P.,Cheng, W.W.L. (deposition date: 2022-06-06, release date: 2022-11-23, Last modification date: 2024-06-12)

Primary citation

Petroff II, J.T.,Dietzen, N.M.,Santiago-McRae, E.,Deng, B.,Washington, M.S.,Chen, L.J.,Trent Moreland, K.,Deng, Z.,Rau, M.,Fitzpatrick, J.A.J.,Yuan, P.,Joseph, T.T.,Henin, J.,Brannigan, G.,Cheng, W.W.L.
Open-channel structure of a pentameric ligand-gated ion channel reveals a mechanism of leaflet-specific phospholipid modulation.
Nat Commun, 13:7017-7017, 2022

PubMed Abstract: Pentameric ligand-gated ion channels (pLGICs) mediate synaptic transmission and are sensitive to their lipid environment. The mechanism of phospholipid modulation of any pLGIC is not well understood. We demonstrate that the model pLGIC, ELIC (Erwinia ligand-gated ion channel), is positively modulated by the anionic phospholipid, phosphatidylglycerol, from the outer leaflet of the membrane. To explore the mechanism of phosphatidylglycerol modulation, we determine a structure of ELIC in an open-channel conformation. The structure shows a bound phospholipid in an outer leaflet site, and structural changes in the phospholipid binding site unique to the open-channel. In combination with streamlined alchemical free energy perturbation calculations and functional measurements in asymmetric liposomes, the data support a mechanism by which an anionic phospholipid stabilizes the activated, open-channel state of a pLGIC by specific, state-dependent binding to this site.

PubMed: 36385237
DOI: 10.1038/s41467-022-34813-5

Experimental method

ELECTRON MICROSCOPY (3.47 Å)