8D5N

Crystal structure of Ld-HF10

Summary for 8D5N

• Entry DOI: 10.2210/pdb8d5n/pdb
• Descriptor: H-2 class I histocompatibility antigen, L-D alpha chain, Dense granule protein 6, HF10 peptide, Beta-2-microglobulin, ... (8 entities in total)
• Functional Keywords: mhci, antigen, cd8 t cell, immune system
• Biological source: Mus musculus (Mouse); More
• Total number of polymer chains: 6
• Total formula weight: 92725.36

Authors

Wang, Y.,Dai, S. (deposition date: 2022-06-05, release date: 2022-09-14, Last modification date: 2024-10-23)

Primary citation

Wang, Y.,Tsitsiklis, A.,Devoe, S.,Gao, W.,Chu, H.H.,Zhang, Y.,Li, W.,Wong, W.K.,Deane, C.M.,Neau, D.,Slansky, J.E.,Thomas, P.G.,Robey, E.A.,Dai, S.
Peptide Centric V beta Specific Germline Contacts Shape a Specialist T Cell Response.
Front Immunol, 13:847092-847092, 2022

PubMed Abstract: Certain CD8 T cell responses are particularly effective at controlling infection, as exemplified by elite control of HIV in individuals harboring HLA-B57. To understand the structural features that contribute to CD8 T cell elite control, we focused on a strongly protective CD8 T cell response directed against a parasite-derived peptide (HF10) presented by an atypical MHC-I molecule, H-2Ld. This response exhibits a focused TCR repertoire dominated by Vβ2, and a representative TCR (TG6) in complex with Ld-HF10 reveals an unusual structure in which both MHC and TCR contribute extensively to peptide specificity, along with a parallel footprint of TCR on its pMHC ligand. The parallel footprint is a common feature of Vβ2-containing TCRs and correlates with an unusual Vα-Vβ interface, CDR loop conformations, and Vβ2-specific germline contacts with peptides. Vβ2 and Ld may represent "specialist" components for antigen recognition that allows for particularly strong and focused T cell responses.

PubMed: 35967379
DOI: 10.3389/fimmu.2022.847092

Experimental method

X-RAY DIFFRACTION (1.8 Å)