8D32

Mycobacterium tuberculosis pduO-type ATP:cobalamin adenosyltransferase bound to 5-deoxyadenosylrhodibalamin and PPPi

8D32 の概要

• エントリーDOI: 10.2210/pdb8d32/pdb
• 分子名称: Corrinoid adenosyltransferase, [5-(5,6-dimethyl-1H-benzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl 1-{3-[3,14,19-tris(2-amino-2-oxoethyl)-8,13,18-tris(3-amino-3-oxopropyl)-1,4,6,9,9,14,16,19-octamethyl-20,21,22,23-tetraazapentacyclo[15.2.1.1~2,5~.1~7,10~.1~12,15~]tricosa-5(23),6,10,12(21),15,17(20)-hexaen-4-yl-kappa~4~N~20~,N~21~,N~22~,N~23~]propanamido}propan-2-yl hydrogenato phosphate]rhodium (non-preferred name), 5'-DEOXYADENOSINE, ... (6 entities in total)
• 機能のキーワード: chaperone, b12 trafficking, transferase, rhodium
• 由来する生物種: Mycobacterium tuberculosis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22650.51

構造登録者

Mascarenhas, R.N.,Ruetz, M.,Koutmos, M.,Banerjee, R. (登録日: 2022-05-31, 公開日: 2023-05-10, 最終更新日: 2024-11-20)

主引用文献

Ruetz, M.,Mascarenhas, R.,Widner, F.,Kieninger, C.,Koutmos, M.,Krautler, B.,Banerjee, R.
A Noble Metal Substitution Leads to B 12 Cofactor Mimicry by a Rhodibalamin.
Biochemistry, 63:1955-1962, 2024

PubMed Abstract: In mammals, cobalamin is an essential cofactor that is delivered by a multitude of chaperones in an elaborate trafficking pathway to two client enzymes, methionine synthase and methylmalonyl-CoA mutase (MMUT). Rhodibalamins, the rhodium analogs of cobalamins, have been described as antimetabolites due to their ability to inhibit bacterial growth. In this study, we have examined the reactivity of adenosylrhodibalamin (AdoRhbl) with two key human chaperones, MMACHC (also known as CblC) and adenosyltransferase (MMAB, also known as ATR), and with the human and MMUT. We demonstrate that while AdoRhbl binds tightly to all four proteins, the Rh-carbon bond is resistant to homolytic (on MMAB and MMUT) as well as heterolytic (on MMACHC) rupture. On the other hand, MMAB catalyzes Rh-carbon bond formation, converting rhodi(I)balamin in the presence of ATP to AdoRhbl. We report the first crystal structure of a rhodibalamin (AdoRhbl) bound to a B protein, i.e., MMAB, in the presence of triphosphate, which shows a weakened but intact Rh-carbon bond. The structure provides insights into how MMAB cleaves the corresponding Co-carbon bond in a sacrificial homolytic reaction that purportedly functions as a cofactor sequestration strategy. Collectively, the study demonstrates that while the noble metal substitution of cobalt by rhodium sets up structural mimicry, it compromises chemistry, which could be exploited for targeting human and bacterial B chaperones and enzymes.

PubMed: 39012171
DOI: 10.1021/acs.biochem.4c00216

実験手法

X-RAY DIFFRACTION (1.85 Å)