8D2L

Structure of Acidothermus cellulolyticus Cas9 ternary complex (Cleavage Intermediate 1)

Summary for 8D2L

• Entry DOI: 10.2210/pdb8d2l/pdb
• Related: 8DTK
• EMDB information: 27141 27142
• Descriptor: CRISPR-associated endonuclease, Csn1 family, Single guide RNA (106-MER), DNA target strand (5'-D(*AP*GP*CP*TP*TP*GP*GP*TP*GP*TP*AP*TP*A)-3'), ... (8 entities in total)
• Functional Keywords: cas9, accas9, crispr, rna binding protein, rna binding protein-dna-rna complex, rna binding protein/dna/rna
• Biological source: Acidothermus cellulolyticus 11B; More
• Total number of polymer chains: 6
• Total formula weight: 178199.97

Authors

Rai, J.,Das, A.,Li, H. (deposition date: 2022-05-30, release date: 2023-12-20)

• Primary citation: Das, A.,Rai, J.,Roth, M.O.,Shu, Y.,Medina, M.L.,Barakat, M.R.,Li, H.; Coupled catalytic states and the role of metal coordination in Cas9; Nat Catal, 6:969-977, 2023; DOI: 10.1038/s41929-023-01031-1

Experimental method

ELECTRON MICROSCOPY (2.21 Å)