8D0U

Human FUT9 bound to GDP

8D0U の概要

• エントリーDOI: 10.2210/pdb8d0u/pdb
• 関連するPDBエントリー: 8D0O 8D0P 8D0Q 8D0R 8D0S
• 分子名称: 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase 9, alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, GUANOSINE-5'-DIPHOSPHATE, ... (6 entities in total)
• 機能のキーワード: glycosyltransferase, alpha-(1, 3)-fucosyltransferase, gt 10, gt-b fold, inverting mechanism, transferase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 39739.11

構造登録者

Kadirvelraj, R.,Wood, Z.A. (登録日: 2022-05-26, 公開日: 2023-05-24, 最終更新日: 2024-11-13)

主引用文献

Kadirvelraj, R.,Boruah, B.M.,Wang, S.,Chapla, D.,Huang, C.,Ramiah, A.,Hudson, K.L.,Prudden, A.R.,Boons, G.J.,Withers, S.G.,Wood, Z.A.,Moremen, K.W.
Structural basis for Lewis antigen synthesis by the alpha 1,3-fucosyltransferase FUT9.
Nat.Chem.Biol., 19:1022-1030, 2023

PubMed Abstract: Mammalian cell surface and secreted glycoproteins exhibit remarkable glycan structural diversity that contributes to numerous physiological and pathogenic interactions. Terminal glycan structures include Lewis antigens synthesized by a collection of α1,3/4-fucosyltransferases (CAZy GT10 family). At present, the only available crystallographic structure of a GT10 member is that of the Helicobacter pylori α1,3-fucosyltransferase, but mammalian GT10 fucosyltransferases are distinct in sequence and substrate specificity compared with the bacterial enzyme. Here, we determined crystal structures of human FUT9, an α1,3-fucosyltransferase that generates Lewis and Lewis antigens, in complex with GDP, acceptor glycans, and as a FUT9-donor analog-acceptor Michaelis complex. The structures reveal substrate specificity determinants and allow prediction of a catalytic model supported by kinetic analyses of numerous active site mutants. Comparisons with other GT10 fucosyltransferases and GT-B fold glycosyltransferases provide evidence for modular evolution of donor- and acceptor-binding sites and specificity for Lewis antigen synthesis among mammalian GT10 fucosyltransferases.

PubMed: 37202521
DOI: 10.1038/s41589-023-01345-y

実験手法

X-RAY DIFFRACTION (1.29 Å)