8CXK
Structure of the C. elegans HIM-3 R93Y mutant
Summary for 8CXK
| Entry DOI | 10.2210/pdb8cxk/pdb |
| Descriptor | HORMA domain-containing protein (2 entities in total) |
| Functional Keywords | meiotic hormad, pch-2, horma domain, hop1, peptide binding protein |
| Biological source | Caenorhabditis elegans |
| Total number of polymer chains | 1 |
| Total formula weight | 33172.81 |
| Authors | Ego, K.M.,Russo, A.,Giacopazzi, S.,Deshong, A.,Menon, M.,Ortiz, V.,Bhalla, N.,Corbett, K.D. (deposition date: 2022-05-21, release date: 2023-05-17, Last modification date: 2023-10-25) |
| Primary citation | Russo, A.E.,Giacopazzi, S.,Deshong, A.,Menon, M.,Ortiz, V.,Ego, K.M.,Corbett, K.D.,Bhalla, N. The conserved AAA ATPase PCH-2 distributes its regulation of meiotic prophase events through multiple meiotic HORMADs in C. elegans. Plos Genet., 19:e1010708-e1010708, 2023 Cited by PubMed Abstract: During meiotic prophase, the essential events of homolog pairing, synapsis, and recombination are coordinated with meiotic progression to promote fidelity and prevent aneuploidy. The conserved AAA+ ATPase PCH-2 coordinates these events to guarantee crossover assurance and accurate chromosome segregation. How PCH-2 accomplishes this coordination is poorly understood. Here, we provide evidence that PCH-2 decelerates pairing, synapsis and recombination in C. elegans by remodeling meiotic HORMADs. We propose that PCH-2 converts the closed versions of these proteins, which drive these meiotic prophase events, to unbuckled conformations, destabilizing interhomolog interactions and delaying meiotic progression. Further, we find that PCH-2 distributes this regulation among three essential meiotic HORMADs in C. elegans: PCH-2 acts through HTP-3 to regulate pairing and synapsis, HIM-3 to promote crossover assurance, and HTP-1 to control meiotic progression. In addition to identifying a molecular mechanism for how PCH-2 regulates interhomolog interactions, our results provide a possible explanation for the expansion of the meiotic HORMAD family as a conserved evolutionary feature of meiosis. Taken together, our work demonstrates that PCH-2's remodeling of meiotic HORMADs has functional consequences for the rate and fidelity of homolog pairing, synapsis, recombination and meiotic progression, ensuring accurate meiotic chromosome segregation. PubMed: 37058535DOI: 10.1371/journal.pgen.1010708 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.3 Å) |
Structure validation
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