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8CXK

Structure of the C. elegans HIM-3 R93Y mutant

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsALS BEAMLINE 8.3.1
Synchrotron siteALS
Beamline8.3.1
Temperature [K]100
Detector technologyPIXEL
Collection date2018-09-14
DetectorDECTRIS PILATUS 6M
Wavelength(s)1.1158
Spacegroup nameP 61
Unit cell lengths73.234, 73.234, 110.816
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution63.420 - 1.300
R-factor0.1449
Rwork0.144
R-free0.16390
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)4trk
RMSD bond length0.021
RMSD bond angle1.579
Data reduction softwareXDS
Data scaling softwareAimless
Phasing softwarePHASER
Refinement softwarePHENIX (1.20.1_4487)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]70.0001.380
High resolution limit [Å]1.3001.300
Rmerge0.0542.027
Rmeas0.0562.081
Number of reflections8228013303
<I/σ(I)>27.38
Completeness [%]100.0100
Redundancy19.819.7
CC(1/2)1.0000.677
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP2931.4-1.6 M sodium malonate pH 6.5-7.0

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