8CXK

Structure of the C. elegans HIM-3 R93Y mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.1158
Spacegroup name	P 61
Unit cell lengths	73.234, 73.234, 110.816
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	63.420 - 1.300
R-factor	0.1449
Rwork	0.144
R-free	0.16390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4trk
RMSD bond length	0.021
RMSD bond angle	1.579
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.000	1.380
High resolution limit [Å]	1.300	1.300
Rmerge	0.054	2.027
Rmeas	0.056	2.081
Number of reflections	82280	13303
<I/σ(I)>	27.38
Completeness [%]	100.0	100
Redundancy	19.8	19.7
CC(1/2)	1.000	0.677

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.4-1.6 M sodium malonate pH 6.5-7.0