8CXK
Structure of the C. elegans HIM-3 R93Y mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.1158 |
Spacegroup name | P 61 |
Unit cell lengths | 73.234, 73.234, 110.816 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 63.420 - 1.300 |
R-factor | 0.1449 |
Rwork | 0.144 |
R-free | 0.16390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4trk |
RMSD bond length | 0.021 |
RMSD bond angle | 1.579 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.000 | 1.380 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.054 | 2.027 |
Rmeas | 0.056 | 2.081 |
Number of reflections | 82280 | 13303 |
<I/σ(I)> | 27.38 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 19.8 | 19.7 |
CC(1/2) | 1.000 | 0.677 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.4-1.6 M sodium malonate pH 6.5-7.0 |