8CXK

Structure of the C. elegans HIM-3 R93Y mutant

8CXK の概要

• エントリーDOI: 10.2210/pdb8cxk/pdb
• 分子名称: HORMA domain-containing protein (2 entities in total)
• 機能のキーワード: meiotic hormad, pch-2, horma domain, hop1, peptide binding protein
• 由来する生物種: Caenorhabditis elegans
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 33172.81

構造登録者

Ego, K.M.,Russo, A.,Giacopazzi, S.,Deshong, A.,Menon, M.,Ortiz, V.,Bhalla, N.,Corbett, K.D. (登録日: 2022-05-21, 公開日: 2023-05-17, 最終更新日: 2023-10-25)

主引用文献

Russo, A.E.,Giacopazzi, S.,Deshong, A.,Menon, M.,Ortiz, V.,Ego, K.M.,Corbett, K.D.,Bhalla, N.
The conserved AAA ATPase PCH-2 distributes its regulation of meiotic prophase events through multiple meiotic HORMADs in C. elegans.
Plos Genet., 19:e1010708-e1010708, 2023

PubMed Abstract: During meiotic prophase, the essential events of homolog pairing, synapsis, and recombination are coordinated with meiotic progression to promote fidelity and prevent aneuploidy. The conserved AAA+ ATPase PCH-2 coordinates these events to guarantee crossover assurance and accurate chromosome segregation. How PCH-2 accomplishes this coordination is poorly understood. Here, we provide evidence that PCH-2 decelerates pairing, synapsis and recombination in C. elegans by remodeling meiotic HORMADs. We propose that PCH-2 converts the closed versions of these proteins, which drive these meiotic prophase events, to unbuckled conformations, destabilizing interhomolog interactions and delaying meiotic progression. Further, we find that PCH-2 distributes this regulation among three essential meiotic HORMADs in C. elegans: PCH-2 acts through HTP-3 to regulate pairing and synapsis, HIM-3 to promote crossover assurance, and HTP-1 to control meiotic progression. In addition to identifying a molecular mechanism for how PCH-2 regulates interhomolog interactions, our results provide a possible explanation for the expansion of the meiotic HORMAD family as a conserved evolutionary feature of meiosis. Taken together, our work demonstrates that PCH-2's remodeling of meiotic HORMADs has functional consequences for the rate and fidelity of homolog pairing, synapsis, recombination and meiotic progression, ensuring accurate meiotic chromosome segregation.

PubMed: 37058535
DOI: 10.1371/journal.pgen.1010708

実験手法

X-RAY DIFFRACTION (1.3 Å)