8CX6
TPX2 Minimal Active Domain on Microtubules
Summary for 8CX6
| Entry DOI | 10.2210/pdb8cx6/pdb |
| NMR Information | BMRB: 31025 |
| Descriptor | Targeting protein for Xklp2-A (1 entity in total) |
| Functional Keywords | spindle assembly factor, branching microtubule nucleation, cell cycle |
| Biological source | Xenopus laevis (African clawed frog) |
| Total number of polymer chains | 1 |
| Total formula weight | 28443.77 |
| Authors | Guo, C.,Alfaro-Aco, R.,Russell, R.,Zhang, C.,Petry, S.,Polenova, T. (deposition date: 2022-05-19, release date: 2023-06-28, Last modification date: 2024-05-15) |
| Primary citation | Guo, C.,Alfaro-Aco, R.,Zhang, C.,Russell, R.W.,Petry, S.,Polenova, T. Structural basis of protein condensation on microtubules underlying branching microtubule nucleation. Nat Commun, 14:3682-3682, 2023 Cited by PubMed Abstract: Targeting protein for Xklp2 (TPX2) is a key factor that stimulates branching microtubule nucleation during cell division. Upon binding to microtubules (MTs), TPX2 forms condensates via liquid-liquid phase separation, which facilitates recruitment of microtubule nucleation factors and tubulin. We report the structure of the TPX2 C-terminal minimal active domain (TPX2) on the microtubule lattice determined by magic-angle-spinning NMR. We demonstrate that TPX2 forms a co-condensate with soluble tubulin on microtubules and binds to MTs between two adjacent protofilaments and at the intersection of four tubulin heterodimers. These interactions stabilize the microtubules and promote the recruitment of tubulin. Our results reveal that TPX2 is disordered in solution and adopts a folded structure on MTs, indicating that TPX2 undergoes structural changes from unfolded to folded states upon binding to microtubules. The aromatic residues form dense interactions in the core, which stabilize folding of TPX2 on microtubules. This work informs on how the phase-separated TPX2 behaves on microtubules and represents an atomic-level structural characterization of a protein that is involved in a condensate on cytoskeletal filaments. PubMed: 37344496DOI: 10.1038/s41467-023-39176-z PDB entries with the same primary citation |
| Experimental method | SOLID-STATE NMR |
Structure validation
Download full validation report
