8CR2

Homo sapiens Get1/Get2 heterotetramer (a3' deletion variant) in complex with a Get3 dimer

8CR2 の概要

• エントリーDOI: 10.2210/pdb8cr2/pdb
• EMDBエントリー: 16802
• 分子名称: ATPase ASNA1, Guided entry of tail-anchored proteins factor CAMLG,Guided entry of tail-anchored proteins factor 1, ZINC ION (3 entities in total)
• 機能のキーワード: membrane protein insertion, get pathway, tail anchored membrane protein, membrane protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 149216.39

構造登録者

McDowell, M.A.,Heimes, M.,Wild, K.,Sinning, I. (登録日: 2023-03-07, 公開日: 2023-11-29, 最終更新日: 2024-10-09)

主引用文献

McDowell, M.A.,Heimes, M.,Enkavi, G.,Farkas, A.,Saar, D.,Wild, K.,Schwappach, B.,Vattulainen, I.,Sinning, I.
The GET insertase exhibits conformational plasticity and induces membrane thinning.
Nat Commun, 14:7355-7355, 2023

PubMed Abstract: The eukaryotic guided entry of tail-anchored proteins (GET) pathway mediates the biogenesis of tail-anchored (TA) membrane proteins at the endoplasmic reticulum. In the cytosol, the Get3 chaperone captures the TA protein substrate and delivers it to the Get1/Get2 membrane protein complex (GET insertase), which then inserts the substrate via a membrane-embedded hydrophilic groove. Here, we present structures, atomistic simulations and functional data of human and Chaetomium thermophilum Get1/Get2/Get3. The core fold of the GET insertase is conserved throughout eukaryotes, whilst thinning of the lipid bilayer occurs in the vicinity of the hydrophilic groove to presumably lower the energetic barrier of membrane insertion. We show that the gating interaction between Get2 helix α3' and Get3 drives conformational changes in both Get3 and the Get1/Get2 membrane heterotetramer. Thus, we provide a framework to understand the conformational plasticity of the GET insertase and how it remodels its membrane environment to promote substrate insertion.

PubMed: 37963916
DOI: 10.1038/s41467-023-42867-2

実験手法

ELECTRON MICROSCOPY (4.2 Å)