8CMY

Structure of the Cyanobium sp. PCC 7001 determined with C1 symmetry

Replaces:  8AFT

Summary for 8CMY

• Entry DOI: 10.2210/pdb8cmy/pdb
• EMDB information: 15409
• Descriptor: Ribulose bisphosphate carboxylase large chain, Ribulose bisphosphate carboxylase small chain, 2-CARBOXYARABINITOL-1,5-DIPHOSPHATE, ... (4 entities in total)
• Functional Keywords: carboxysome, carbon fixation, cyanobacteria, photosynthesis
• Biological source: Cyanobium; More
• Total number of polymer chains: 16
• Total formula weight: 525855.24

Authors

Evans, S.L.,Bergeron, J.R.C. (deposition date: 2023-02-21, release date: 2023-03-22, Last modification date: 2025-10-01)

Primary citation

Evans, S.L.,Al-Hazeem, M.M.J.,Mann, D.,Smetacek, N.,Beavil, A.J.,Sun, Y.,Chen, T.,Dykes, G.F.,Liu, L.N.,Bergeron, J.R.C.
Single-particle cryo-EM analysis of the shell architecture and internal organization of an intact alpha-carboxysome.
Structure, 31:677-, 2023

PubMed Abstract: Carboxysomes are proteinaceous bacterial microcompartments that sequester the key enzymes for carbon fixation in cyanobacteria and some proteobacteria. They consist of a virus-like icosahedral shell, encapsulating several enzymes, including ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO), responsible for the first step of the Calvin-Benson-Bassham cycle. Despite their significance in carbon fixation and great bioengineering potentials, the structural understanding of native carboxysomes is currently limited to low-resolution studies. Here, we report the characterization of a native α-carboxysome from a marine cyanobacterium by single-particle cryoelectron microscopy (cryo-EM). We have determined the structure of its RuBisCO enzyme, and obtained low-resolution maps of its icosahedral shell, and of its concentric interior organization. Using integrative modeling approaches, we have proposed a complete atomic model of an intact carboxysome, providing insight into its organization and assembly. This is critical for a better understanding of the carbon fixation mechanism and toward repurposing carboxysomes in synthetic biology for biotechnological applications.

PubMed: 37015227
DOI: 10.1016/j.str.2023.03.008

Experimental method

ELECTRON MICROSCOPY (3.79 Å)