Loading
PDBj
MenuPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

8CCY

Human heparan sulfate N-deacetylase-N-sulfotransferase 1 in complex with calcium and 3'-phosphoadenosine-5'-phosphosulfate

Summary for 8CCY
Entry DOI10.2210/pdb8ccy/pdb
Related1NST
EMDB information16564
DescriptorBifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1, ADENOSINE-3'-5'-DIPHOSPHATE, CALCIUM ION, ... (4 entities in total)
Functional Keywordsdeacetylase, sulfotransferase, heparan sulfate, carbohydrate, glycosaminoglycan
Biological sourceHomo sapiens (human)
Total number of polymer chains1
Total formula weight92914.82
Authors
Mycroft-West, C.J.,Wu, L. (deposition date: 2023-01-28, release date: 2024-02-07, Last modification date: 2024-11-06)
Primary citationMycroft-West, C.J.,Abdelkarim, S.,Duyvesteyn, H.M.E.,Gandhi, N.S.,Skidmore, M.A.,Owens, R.J.,Wu, L.
Structural and mechanistic characterization of bifunctional heparan sulfate N-deacetylase-N-sulfotransferase 1.
Nat Commun, 15:1326-1326, 2024
Cited by
PubMed Abstract: Heparan sulfate (HS) polysaccharides are major constituents of the extracellular matrix, which are involved in myriad structural and signaling processes. Mature HS polysaccharides contain complex, non-templated patterns of sulfation and epimerization, which mediate interactions with diverse protein partners. Complex HS modifications form around initial clusters of glucosamine-N-sulfate (GlcNS) on nascent polysaccharide chains, but the mechanistic basis underpinning incorporation of GlcNS itself into HS remains unclear. Here, we determine cryo-electron microscopy structures of human N-deacetylase-N-sulfotransferase (NDST)1, the bifunctional enzyme primarily responsible for initial GlcNS modification of HS. Our structures reveal the architecture of both NDST1 deacetylase and sulfotransferase catalytic domains, alongside a non-catalytic N-terminal domain. The two catalytic domains of NDST1 adopt a distinct back-to-back topology that limits direct cooperativity. Binding analyses, aided by activity-modulating nanobodies, suggest that anchoring of the substrate at the sulfotransferase domain initiates the NDST1 catalytic cycle, providing a plausible mechanism for cooperativity despite spatial domain separation. Our data shed light on key determinants of NDST1 activity, and describe tools to probe NDST1 function in vitro and in vivo.
PubMed: 38351061
DOI: 10.1038/s41467-024-45419-4
PDB entries with the same primary citation
Experimental method
ELECTRON MICROSCOPY (2.7 Å)
Structure validation

227561

PDB entries from 2024-11-20

PDB statisticsPDBj update infoContact PDBjnumon