8C4C

F-actin decorated by SipA497-669

Summary for 8C4C

• Entry DOI: 10.2210/pdb8c4c/pdb
• EMDB information: 16424
• Descriptor: Actin, alpha skeletal muscle, Cell invasion protein SipA, ADENOSINE-5'-DIPHOSPHATE, ... (5 entities in total)
• Functional Keywords: salmonella invasion, cell invasion
• Biological source: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2; More
• Total number of polymer chains: 12
• Total formula weight: 417826.02

Authors

Yuan, B.,Wald, J.,Marlovits, T.C. (deposition date: 2023-01-03, release date: 2024-01-17)

Primary citation

Yuan, B.,Scholz, J.,Wald, J.,Thuenauer, R.,Hennell James, R.,Ellenberg, I.,Windhorst, S.,Faix, J.,Marlovits, T.C.
Structural basis for subversion of host cell actin cytoskeleton during Salmonella infection.
Sci Adv, 9:eadj5777-eadj5777, 2023

PubMed Abstract: Secreted bacterial type III secretion system (T3SS) proteins are essential for successful infection by many human pathogens. Both T3SS translocator SipC and effector SipA are critical for infection by subversion of the host cell cytoskeleton, but the precise molecular interplay between them remains unknown. Here, using cryo-electron microscopy, we show that SipA binds along the F-actin grooves with a unique binding pattern. SipA stabilizes F-actin through charged interface residues and appears to prevent inorganic phosphate release through closure of the "back door" of adenosine 5'-triphosphate pocket. We also show that SipC enhances the binding of SipA to F-actin, thus demonstrating that a sequential presence of T3SS proteins in host cells is associated with a sequence of infection events-starting with actin nucleation, filament growth, and stabilization. Together, our data explain the coordinated interplay of a precisely tuned and highly effective mechanism during infection and provide a blueprint for interfering with effectors acting on actin.

PubMed: 38064550
DOI: 10.1126/sciadv.adj5777

Experimental method

ELECTRON MICROSCOPY (2.7 Å)