8C1A

SARS-CoV-2 NSP3 macrodomain in complex with aztreonam

Summary for 8C1A

• Entry DOI: 10.2210/pdb8c1a/pdb
• Descriptor: Replicase polyprotein 1ab, aztreonam, 1,2-ETHANEDIOL, ... (4 entities in total)
• Functional Keywords: macrodomain, adp-ribose, sars-cov-2, viral protein
• Biological source: Severe acute respiratory syndrome coronavirus 2
• Total number of polymer chains: 2
• Total formula weight: 36855.03

Authors

Schuller, M.,Ahel, I. (deposition date: 2022-12-20, release date: 2023-03-08, Last modification date: 2024-02-07)

Primary citation

Schuller, M.,Zarganes-Tzitzikas, T.,Bennett, J.,De Cesco, S.,Fearon, D.,von Delft, F.,Fedorov, O.,Brennan, P.E.,Ahel, I.
Discovery and Development Strategies for SARS-CoV-2 NSP3 Macrodomain Inhibitors.
Pathogens, 12:-, 2023

PubMed Abstract: The worldwide public health and socioeconomic consequences caused by the COVID-19 pandemic highlight the importance of increasing preparedness for viral disease outbreaks by providing rapid disease prevention and treatment strategies. The NSP3 macrodomain of coronaviruses including SARS-CoV-2 is among the viral protein repertoire that was identified as a potential target for the development of antiviral agents, due to its critical role in viral replication and consequent pathogenicity in the host. By combining virtual and biophysical screening efforts, we discovered several experimental small molecules and FDA-approved drugs as inhibitors of the NSP3 macrodomain. Analogue characterisation of the hit matter and crystallographic studies confirming binding modes, including that of the antibiotic compound aztreonam, to the active site of the macrodomain provide valuable structure-activity relationship information that support current approaches and open up new avenues for NSP3 macrodomain inhibitor development.

PubMed: 36839595
DOI: 10.3390/pathogens12020324

Experimental method

X-RAY DIFFRACTION (1.9 Å)