8C0T

NRS 1.2: Fluorescent Sensors for Imaging Interstitial Calcium

Summary for 8C0T

• Entry DOI: 10.2210/pdb8c0t/pdb
• Descriptor: mNeonGreen,Optimized Ratiometric Calcium Sensor, CALCIUM ION, SULFATE ION, ... (4 entities in total)
• Functional Keywords: fluorescent sensors for imaging interstitial calcium, biosynthetic protein
• Biological source: Branchiostoma lanceolatum (Common lancelet, Amphioxus lanceolatum)
• Total number of polymer chains: 1
• Total formula weight: 34584.06

Authors

Basquin, J.,Griesbeck, O.,Valiente-Gabioud, A. (deposition date: 2022-12-19, release date: 2023-11-15, Last modification date: 2024-10-23)

Primary citation

Valiente-Gabioud, A.A.,Garteizgogeascoa Suner, I.,Idziak, A.,Fabritius, A.,Basquin, J.,Angibaud, J.,Nagerl, U.V.,Singh, S.P.,Griesbeck, O.
Fluorescent sensors for imaging of interstitial calcium.
Nat Commun, 14:6220-6220, 2023

PubMed Abstract: Calcium in interstitial fluids is central to systemic physiology and a crucial ion pool for entry into cells through numerous plasma membrane channels. Its study has been limited by the scarcity of methods that allow monitoring in tight inter-cell spaces of living tissues. Here we present high performance ultra-low affinity genetically encoded calcium biosensors named GreenT-ECs. GreenT-ECs combine large fluorescence changes upon calcium binding and binding affinities (Kds) ranging from 0.8 mM to 2.9 mM, making them tuned to calcium concentrations in extracellular organismal fluids. We validated GreenT-ECs in rodent hippocampal neurons and transgenic zebrafish in vivo, where the sensors enabled monitoring homeostatic regulation of tissue interstitial calcium. GreenT-ECs may become useful for recording very large calcium transients and for imaging calcium homeostasis in inter-cell structures in live tissues and organisms.

PubMed: 37798285
DOI: 10.1038/s41467-023-41928-w

Experimental method

X-RAY DIFFRACTION (1.28 Å)