8BZQ
Beta-1,4-D-endoglucanase Cel45A from Gloeophyllum trabeum
Summary for 8BZQ
| Entry DOI | 10.2210/pdb8bzq/pdb |
| Descriptor | Endoglucanase V-like protein (2 entities in total) |
| Functional Keywords | endoglucanase, gtcel45a, gh45 family, cellulase, brown rot, hydrolase |
| Biological source | Gloeophyllum trabeum ATCC 11539 |
| Total number of polymer chains | 1 |
| Total formula weight | 18392.84 |
| Authors | Okmane, L.,Fitkin, L.,Stahlberg, J. (deposition date: 2022-12-15, release date: 2023-11-22, Last modification date: 2024-10-23) |
| Primary citation | Okmane, L.,Fitkin, L.,Sandgren, M.,Stahlberg, J. The first crystal structure of a family 45 glycoside hydrolase from a brown-rot fungus, Gloeophyllum trabeum GtCel45A. Febs Open Bio, 14:505-514, 2024 Cited by PubMed Abstract: Here we describe the first crystal structure of a beta-1,4-endoglucanase from a brown-rot fungus, Gloeophyllum trabeum GtCel45A, which belongs to subfamily C of glycoside hydrolase family 45 (GH45). GtCel45A is ~ 18 kDa in size and the crystal structure contains 179 amino acids. The structure is refined at 1.30 Å resolution and R 0.18. The enzyme consists of a single catalytic module folded into a six-stranded double-psi beta-barrel domain surrounded by long loops. GtCel45A is very similar in sequence (82% identity) and structure to PcCel45A from the white-rot fungus Phanerochaete chrysosporium. Surprisingly though, initial hydrolysis of barley beta-glucan was almost twice as fast in GtCel45A as compared to PcCel45A. PubMed: 38311343DOI: 10.1002/2211-5463.13774 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.3 Å) |
Structure validation
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